Long noncoding RNAs (lncRNAs) are a type of RNA that are longer than 200 nucleotides with no apparent protein-coding potentials. To date, lncRNAs have been shown to have diverse functional roles in many fundamental cellular processes, and they also represent important components of ongoing research in many fields. Typically, the first step is identifying or sequencing IncRNAs within the specific tissues of interest. The next step is usually to apply sequencing techniques to the purified RNAs. The final step is the functional characterization of the new IncRNAs, which may involve perturbing transcription through CRISPR-mediated deletions and overexpression of IncRNAs by transgenesis or transcription.

Lifeasible, as a leading global company, is committed to helping our customers achieve effective and successful research. We provide analysis services for plant lncRNA functions, including acquisition-of-function, loss-of-function, and other knockdown analyses. In addition, we deliver reliable results and reports on time to our customers worldwide.

Plant lncRNA Gain-of-Function Analysis

• Gain-of-function can result in altered gene products with new molecular functions or new gene expression patterns. In principle, full-length lncRNAs are targeted to be cloned into expression vectors for overexpression of lncRNAs. However, some lncRNAs are very large or full-length lncRNAs have not yet been isolated, and different research strategies will be adopted depending on the positioning of the lncRNA on the genome.
• Based on the lncRNA to be overexpressed and the cell line to be transfected, we provide a complete set of technical services, including lncRNA overexpression vector construction, plant transfection, gene expression assay, screening of overexpression plants, phenotyping of transgenic plants, and others.

Fig. 1 Diagram of loss-of-function and gain-of-function.

Plant lncRNA Loss-Of-Function Analysis

• Loss-of-function of lncRNAs is performed by using the RNA interference (RNAi) method, i.e., specific degradation of homologous RNAs mediated by small interfering RNAs (siRNAs) and short hairpin RNAs (shRNAs), which is one of the most prominent and common techniques in lncRNA functional studies.
• Based on the lncRNA gene sequence, we help our customers design and synthesize siRNA or shRNA, transfect with fluorescently labeled siRNA, verify the silencing efficiency of siRNA or shRNA, and subsequent experiments.

Functional Analysis of Plant lncRNA with CRISPR-Cas Technology

• The CRISPR-Cas9 (clustered regulatory interspaced short palindromic repeats/CRISPR-associated protein 9) technology is a breakthrough in genome editing technology. CRISPR loci are transcribed into ncRNA and eventually form a functional complex with Cas9 and further guide the complex to cleave complementary invading DNA.
• We provide a complete and comprehensive functional analysis of plant lncRNA with CRISPR-Cas9 technology, including cloning of the full-length lncRNA, constructing CRISPR/Cas 9 vector, and plant genetic transformation.

Plant lncRNA Other Knockdown Analysis

• The lncRNAs have longer sequences, carry more information, and are more likely to form higher-level structures; therefore, their functions are diverse and specific.
• We also offer other knockdown methods to study the effects of lncRNA on plant growth, development, and stress response, such as CRISPR/Cas9, antisense oligonucleotides, site-reversal, promoter deletion, and others.

Lifeasible is always devoted to providing high-quality and satisfactory service to our customers. If you are interested in our services or have questions, please contact us or make an online inquiry.

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Related Services

• Identification of Plant lncRNA
• Analysis of Plant lncRNA Structure
• Analysis of Plant lncRNA Verification