The formation of different cell fate states is the basis for the formation of multicellular organisms, and epigenetic modifications play a fundamental regulatory role in cell fate determination. Transposable elements (TEs) are a class of DNA that can move freely within the genome. As the main anchors of epigenetic modifications, TEs are also involved in regulating cell fate determination. Lifeasible drives your research in TEs with bioinformatics analyses such as whole genome/transcriptome deep sequencing, chromatin modification characterization, and expression profiling.

TEs testing service

We can provide you with the determination of TEs content, TEs insertion gene location, and specific TEs in the genomes of different species, including CACTA, Mutator, PIF/Harbinger, hAT, Tc1/mariner, and helitron. Species we have successfully assayed include:

Bioinformatics analysis service for simultaneous quantification of gene and TEs expression

• Since TEs are multi-copy repetitive sequences, there are usually thousands of different copies of the same TEs spread throughout the genome, and the sequence between each copy is highly similar. Therefore, it is often difficult to accurately quantify TEs at a single point. To address this problem, and due to the factor that conventional single-cell ATAC-seq (scATAC-seq) has only short sequencing read lengths, we provide a quantitative strategy for TEs at the family level. This strategy ignores genomic positional information and improves the quantitative accuracy of TEs.
• The development of single-cell genomics technologies, represented by scATAC-seq, has made it possible to obtain high-resolution chromatin opening/modification profiles with single-cell accuracy. We were able to effectively compensate for the shortcomings of scATAC-seq data by removing the sparsity and reducing the dimensionality of the data by accumulating TE signals. We could distinguish the major cell types in scATAC-seq data using TE signals alone.

TEs are the most abundant elements in the genome. TEs are important in regulating gene expression by affecting chromatin modification, transcription factor binding, RNA editing, chromatin high-level structure, etc. Lifeasible provides TEs detection and quantitative association analysis services to help you better understand the relationship between non-coding RNAs, TEs, and host genes. Please feel free to contact us for more information.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

For research or industrial use.

Related Services

• Synthesis of Plant Artificial Chromosomes
• Gene Expression Assays
• Analysis of Gene Imprinting
• Epigenome Mapping