Salt stress is one of the main abiotic stresses restricting plant growth and development, and it is also a serious problem restricting crop production and ecological environment construction. Plant salt stress refers to the fact that plants grow in a high-salt environment and are affected by high osmotic potential. In addition to the direct hyperosmotic poisoning effect of salt stress, there is also an indirect oxidative stress effect. Salt stress causes metabolic disorders in plants, causing damage to the plant's redox system. Reactive oxygen species and other substances generated during the metabolic process accumulate in the plant, affecting the plant's photosynthesis and ultimately inhibiting plant growth. Salt stress negatively affects plant growth and triggers adaptive selections of the natural habitats of the plants, causing a serious threat to the environment.

Different plants have different tolerances to salt stress, and the root cause is whether there are related genes, whether the related genes are expressed, and whether the related genes are overexpressed. Long non-coding RNAs (lncRNAs) are a class of RNA molecules that do not code for proteins but play crucial roles in regulating gene expression. Recent studies have shown that plant lncRNAs are involved in the response to salt stress and help plants adapt to adverse conditions.

With years of experience in the industry, our team at Lifeasible consists of highly skilled experts in plant biology and molecular analysis.

Mechanisms of Plant lncRNA Response to Salt Stress

• Ion regulation

We provide analysis services of plant lncRNA in ion regulation by modulating the expression of genes involved in ion transport. Maintaining ion homeostasis is crucial for plant survival under salt stress.

• Reactive oxygen species (ROS) removal

We offer analysis of plant lncRNA in ROS removal by regulating the expression of genes encoding antioxidant enzymes. Salt stress induces the accumulation of ROS, which can cause oxidative damage to plant cells. Plant lncRNAs were found to be involved in scavenging ROS in response to salt stress.

• Phytohormone synthesis

We recognize phytohormones are critical regulators of plant growth and development and stress responses. Therefore, we provide participation of plant lncRNAs in phytohormone synthesis pathways, influencing plant responses to salt stress.

• Signal transduction

We provide analysis of plant lncRNA in signal transduction, which plays a crucial role in transmitting stress signals from the cell membrane to the nucleus, leading to appropriate physiological and molecular responses. Plant lncRNAs are involved in signal transduction pathways, relaying salt stress signals to downstream effectors.

Our Workflow

Lifeasible specializes in providing comprehensive solutions for analyzing plant lncRNAs in response to salt stress. Our service flow includes the following steps.

Fig. 1 Representative examples of how plant lncRNAs respond to salt stresses. (Yang H, et al., 2023)

• Sample collection. We offer expert guidance on collecting plant samples under salt-stress conditions, ensuring optimal sample quality.
• RNA extraction. Our advanced techniques allow for high-quality total RNA extraction, including lncRNAs, from plant samples. We ensure minimal degradation and contamination during the process.
• lncRNA profiling. We utilize state-of-the-art sequencing technologies, such as RNA-seq, to comprehensively profile the expression of plant lncRNAs under salt stress.
• Differential expression analysis. We perform rigorous statistical analysis to identify differentially expressed lncRNAs in response to salt stress, providing insights into their potential roles.
• Functional analysis and validation. We perform functional analysis of differentially expressed lncRNAs, uncovering their involvement in ion regulation, ROS scavenging, phytohormone synthesis, and signal transduction pathways. We also offer experimental services, such as qRT-PCR, to confirm the expression patterns of selected lncRNAs.
• Data interpretation and reporting. A comprehensive report, including all experimental details and analysis results, is presented to the clients.

Lifeasible is proud to be at the forefront of this exciting field, providing high-quality solutions for analyzing plant lncRNAs in response to salt stress and other stress conditions. If you are interested in our services or have questions, please feel free to contact us or make an online inquiry.

Reference

1. Yang H, et al. (2023). "Long Non-Coding RNAs of Plants in Response to Abiotic Stresses and Their Regulating Roles in Promoting Environmental Adaption." Cells. 12 (5), 729.

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