Identification of Coffee Varieties by SRAP Markers

In international trade, coffee is the most valuable commodity after oil. As one of the three major beverages in the world, coffee is consumed by many people and is in high demand. In addition to drinking, coffee can also extract caffeine, which is used as an anesthetic, a cardiac stimulant, and a diuretic. In addition, its pulp contains sugar, which can be used to make alcohol or feed. Therefore, coffee has an extremely high economic value. The identification of coffee varieties has now become a common problem in selecting and breeding high-quality coffee varieties, and the accurate identification of coffee varieties plays a valuable role in protecting and improving the economic value of coffee.

Lifeasible provides Sequence-related amplified polymorphism (SRAP), a novel PCR-based marker system, as a dominant marker, which amplifies specific regions of ORFs (open reading frames) of genes through a unique double primer design. The upstream primer is 17 bp long and extends the specific region of the exon. The downstream primer is 18 bp long and amplifies the intronic and promoter regions. Polymorphism arises due to the different lengths of introns, promoters, and spacer regions in different individuals and species. By constructing DNA fingerprints of coffee varieties, this technique has been widely used to compare coffee variety genetic diversity. It has also been used to identify and protect varieties by comparing coffee genetic diversity.

Application of SRAP markers in coffee varieties identification

• Analysis of genetic diversity of coffee varieties
• Construction of genetic maps
• Markers for important traits
• Cloning of related genes

Features of SRAP markers for coffee variety identification

The unique primer design of SRAP makes it possible to detect the ORFs region of genes. It is a new molecular marker technology that can be PCR-amplified without sequence information. This overcomes the problems of poor reproducibility of RAPD and the disadvantages of complex and expensive AFLP technology and has the following features.

• Convenient and easy to operate
• Low cost and high reliability
• Good reproducibility
• Easy sequencing
• Easy to clone target fragments

Our service workflow

Advantages of our services

• Flexible customized experimental solutions. Innovative experimental design to meet customers' specific research needs.
• Full technical support. Our experts will help you to solve the problems encountered in the progress of your experimental project.
• Strict quality control system. We have a standardized service process to ensure the maximum success rate of experiments.
• Attentive after-sales service. To reduce your worries about the progress of your project.

Lifeasible provides a wide-range of technical services and solutions for plant science research. Our team of biotechnology professionals aims to provide advanced biotechnology to advance plant science research. If you are interested in us, please feel free to contact us.

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For research use only, not intended for any clinical use.

Related Solutions

• Identifying Coffee Varieties by DNA Fingerprinting
• Identify Coffee Varieties with SSR Markers