Meristem Tissue Culture

Meristematic tissues are cells with the ability to divide or differentiate continuously or periodically in specific tissue parts of plants and are the basis for the generation and differentiation of various other tissues. It is characterized by small size, very tightly arranged cells, no cell gaps, relatively large nuclei, no or thin cell walls, and intracellular filled with protoplasm. According to their different locations, they can be divided into three types, apical meristem, lateral meristem, and interstitial meristem.

Leaf meristems of Arabidopsis thaliana.Figure 1. Leaf meristems of Arabidopsis thaliana. (Yasunori I., et al., 2015)

What We Offer

The rapid development of plant tissue culture has aroused the interest of researchers in plant meristematic tissue culture research, based on which Lifeasible has established a complete plant meristematic tissue culture system and plant regeneration system to obtain regenerated plants by tissue culture of different plant species and different tissue organs.

Plant meristem tissue culture service flow - Lifeasible

Lifeasible used the stem tip meristem culture technique to obtain virus-free in vitro seedlings of the target plants. In the culture, we used the basic medium and hormone ratios to select the best medium composition to assist in the rapid propagation of the virus-free seedlings.

  • We have established an efficient plant regeneration system using different plant stem tips as explants with the standard control system in terms of seedling age, activated charcoal, growth regulators, and IBA of sterile seedlings.
  • The sterilization of the material is also the key to the culture process, and we offer two methods of sterilization, the two-step method, and the one-step method.
  • The hormone ratios will also be customized according to your material.

We Do Better

Lifeasible has a strict control system for factors affecting the effect of stem tip culture, such as culture medium, explant size (detoxification effect is negatively correlated with explant size and stem tip viability is positively correlated with stem tip size), storage conditions (light culture vs. dark culture), physiological status of explants (actively growing shoots, better effect of apical shoots than axillary shoots, time to cut shoots), etc. We are based in the plant technology industry with professional and fast technical services, and our strict system and mature platform guarantee the success of your project.

Reference

  1. Yasunori I., et al., “Behavior of Leaf Meristems and Their Modification.” Front. Plant Sci., 2015, 6:1060.

The services provided by Lifeasible cover all aspects of plant research, please contact us to find out how we can help you achieve the next research breakthrough.

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