For multicellular organisms, there is inherent cellular heterogeneity. With the rapid development of single-cell sequencing technologies, our understanding of cell heterogeneity and cell function has been greatly enriched. Lifeasible is committed to providing comprehensive plant single-cell sequencing services to customers worldwide, from plant single-cell transcriptomics sequencing, and data analysis processes to applications.

Advances in Plant Single-cell Sequencing Research

Single-cell transcriptome sequencing is a technique for studying gene expression at the single-cell level that has led to new insights into animal biology, such as the discovery of new cell types and cell type-specific disease processes. However, the application of single-cell methods in plants, fungi, algae, or bacteria (environmental organisms) is more limited, mainly due to the challenges posed by the polysaccharide walls surrounding the cells of these species. Single-cell RNA sequencing (scRNA-seq) is currently the most widely used single-cell transcriptome technology for plant research. ScRNA-seq methods overcome the challenges of aggregated gene expression throughout tissues, enabling the identification of individual cells at high resolution, discovering new cells, and a better understanding of cell uniqueness. In addition, single-cell assay for transposase-accessible chromatin sequencing (scATAC-seq) provides epigenomic analysis that has been increasingly used in plants and is well suited for exploring gene regulation, elucidating regulatory networks, and even classifying cell types at a finer scale. ScRNA-seq and scATAC-seq datasets are highly complementary and can be efficiently combined.

Fig.1. Generation of singe-cell transcriptomic data using microfluidic technology. (Rich-Griffin C et al., 2020)

What We Offer

As a leading plant biotechnology company, Lifeasible offers comprehensive and reliable plant single-cell sequencing services that can help you uncover the transcriptomes of various plant cells isolated from different organs and different species and their responses to environmental stresses. In addition, we deliver reliable results and reports on time to our customers worldwide.

Our laboratory has two major platforms for plant research, scRNA-seq, and scATAC-seq. Our services support single-cell transcriptomics technologies to decipher the biological functions of plant cells, their developmental programs, cell type-specific gene networks, programs that control plant cell responses to environmental stresses, etc. Our single-cell transcriptome sequencing services are designed to provide new perspectives for research in the plant field and help you identify and understand key cell biological processes in complex tissues.

Our plant single-cell sequencing services include:

• Plant Single-cell RNA Sequencing (scRNA-seq)
  Our scRNA-seq platform for plants can comprehensively characterize common and rare cell types and cell states, discover new cell types and reveal how cell types are spatially and developmentally interconnected. We offer droplet-based scRNA-seq and plate-based scRNA-seq technologies for a wide range of species such as Arabidopsis, rice, maize, tomato, poplar, etc. The appropriate technical approach can be selected based on the experimental purpose and the nature of the plant sample. In addition, we use bioinformatics to help you analyze plant scRNA-seq data and ensure reliable results.
• Plant Single-cell Assay for Transposase-Accessible Chromatin Sequencing (scATAC-seq)
  We are developing the scATAC-seq platform for plant epigenomic analysis to help you explore gene regulation, elucidate regulatory networks, and classify cell types. In addition, we use bioinformatics to help you analyze plant scATAC-seq data to ensure reliable results.
• Single-cell Solutions for Plant Research
  Plant research has entered the single-cell era. We provide professional solutions to use scATAC-seq technology in plant research, including constructing cell atlases, identifying rare cell taxa, mining new Marker genes, studying cell developmental dynamics, analyzing gene regulatory networks, studying abiotic stress response mechanisms, conserved and differential analysis, and studying the functions of essential transcription factors. Our scRNA-seq aims to open up new opportunities in plant science and plant stress management.

Service Process

• Preparation of protoplast suspensions.
• Isolation of plant nuclei.
• Plant single-cell sequencing.
• Bioinformatics analysis of plant single-cell transcriptomes.
• A final report with detailed data and figures.

Lifeasible offers professional plant single-cell transcriptomics studies to help you gain a deeper understanding of the role of different cell types in development and the regulatory networks between cells. From single to multiple tissues, we are developing single-cell multiomics co-analyses designed to revolutionize plant science research at an unprecedented level. If you are interested in our services or have some questions, please feel free to contact us or make an online inquiry.

Reference

1. Rich-Griffin C, Stechemesser A, Finch J, et al. Single-cell transcriptomics: a high-resolution avenue for plant functional genomics[J]. Trends in plant science, 2020, 25(2): 186-197.

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For research use only, not intended for any clinical use.

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