The protein hydrolysis process refers to the cutting of proteins into shorter fragments, i.e., peptides. Plant protein hydrolysis is a crucial step before peptide identification and analysis by mass spectrometry and is the basis for plant protein identification and characterization, biomarker discovery, etc. to proceed. Although intact proteins can be studied by mass spectrometry, protein identification is easier using smaller peptides and the coverage of identification can be improved. Therefore, the most common plant proteomics approaches tend to utilize site-specific enzymatic cleavage sites to generate smaller peptide fragments, and smaller mass peptides are more easily separated and characterized by HPLC and coupled mass spectrometry.

What We Offer

• Plant protein hydrolysis services

Lifeasible offers two methods of plant protein hydrolysis, in-gel hydrolysis (protein hydrolysis in a polyacrylamide gel matrix) and in-solution hydrolysis (protein precipitation in chloroform/methanol followed by re-dissolution in urea). Proteins separated by one- or two-dimensional gel electrophoresis can be hydrolyzed directly in-gel. In-gel hydrolysis, which is effective and reproducible, is more laborious and time-consuming. Compared to in-gel hydrolysis, the in-solution hydrolysis method is less laborious and requires less time, but in-solution hydrolysis of proteins, with low protein re-solubility, may lead to some sample loss.

Service Flow

Sample Requirement

• Proteins of natural plant origin
• Fusion and tagged proteins of recombinant origin
• Plant membrane proteins and others

*There is no restriction on plant varieties. For varieties with special requirements or rare varieties, please contact our staff for more information.

We Do Better

Lifeasible offers both in-gel and in-solution hydrolysis strategies for plant proteins. Our hydrolysis services can greatly improve the accuracy and coverage of mass spectrometry analysis, which can facilitate the identification and characterization of plant proteins, discover biomarkers, and contribute to the understanding of plant biological processes. Besides, we offer a complete plant proteomics service covering the entire process of plant proteomics analysis, which can be performed on hydrolyzed samples to provide a one-stop solution to your plant proteomics problems.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

For research use only, not intended for any clinical use.

Related Services

• Plant Protein Gel and Image Analysis Services