In recent years, the global climate has changed abnormally, extreme weather and agricultural meteorological disasters have occurred frequently, and sudden low-temperature stress has become one of the most common abiotic stresses in agricultural production. Cold stress is a common abiotic stress condition that plants encounter during winter or in regions with cold climates. Low-temperature stress is one of the most essential adversity stresses affecting plant growth and crop yield. It is very important to develop low-temperature stress-tolerant plants. Usually, low temperatures will cause mechanical damage to plants. Signal receptors at the membrane level are the first step in receiving stress signals, and then transducing the signals into cells to affect stress response genes, which is very important for improving crop yield and seriously restricting agricultural production.

Long noncoding RNAs (lncRNAs) are expressed in specific tissues and developmental stages of organisms and are specifically expressed in response to environmental signals to regulate various biological processes. lncRNAs play the role of regulators in plant growth and development and environmental stress responses. Plant lncRNAs in response to cold stress are specific long non-coding RNAs expressed by plants in response to cold temperature exposure. These lncRNAs have been shown to play essential roles in regulating gene expression and coordinating the plant's response to cold stress.

At Lifeasible, our team consists of highly skilled and experienced experts in molecular biology and bioinformatics. Additionally, we employ cutting-edge technologies and advanced bioinformatics tools to provide accurate and comprehensive sequencing data analysis.

Mechanisms of Plant lncRNA Response to Cold Stress

• Promoting the transcription of cold-tolerant genes and transcription factors

We provide analysis services for plant lncRNA in response to cold stress by promoting the transcription of cold-tolerant genes and transcription factors. Plant lncRNAs can interact with chromatin modifiers and transcription factors to regulate the expression of cold-responsive genes. Additionally, lncRNAs can directly interact with transcription factors, either stabilizing them or facilitating their binding to the promoters of cold-responsive genes.

• Increasing the activity of antioxidant enzymes

We offer another crucial solution of plant lncRNA to cope with cold stress by increasing the activity of antioxidant enzymes. By enhancing the activity of antioxidant enzymes, lncRNAs protect plant cells from the damaging effects of ROS and improve their tolerance to cold stress.

• Reducing the damage of reactive oxygen species (ROS)

We also offer analysis of plant lncRNAs in directly reducing the damage caused by reactive oxygen species, in addition to increasing the activity of antioxidant enzymes. Certain lncRNAs can act as molecular sponges for ROS, sequestering these harmful molecules and preventing them from interacting with cellular components.

Our Workflow

Fig. 1 Representative examples of how plant lncRNAs respond to cold stresses. (Yang H, et al., 2023)

• Sample collection and RNA isolation. We assist in the proper collection of plant samples under cold-stress conditions. Subsequently, we employ advanced techniques for the isolation of high-quality RNA.
• RNA sequencing. We employ next-generation sequencing to obtain high-throughput data.
• Data analysis and annotation. We employ advanced bioinformatics tools and pipelines to analyze the sequencing data and identify differentially expressed lncRNAs in response to cold stress. We also provide functional annotation and classification of the identified lncRNAs.
• Validation and functional studies. To validate the expression patterns of identified lncRNAs, we offer a range of experimental techniques, including quantitative PCR and Northern blot analysis. Functional studies can also be conducted to elucidate the precise role of lncRNAs in coping with cold stress.

At Lifeasible, we strive to offer tailor-made solutions that meet the specific requirements of our clients, enabling them to gain a deeper understanding of the role of plant lncRNAs in coping with cold stress. If you are interested in our services or have questions, please feel free to contact us or make an online inquiry.

Reference

1. Yang H, et al. (2023). "Long Non-Coding RNAs of Plants in Response to Abiotic Stresses and Their Regulating Roles in Promoting Environmental Adaption." Cells. 12 (5), 729.

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