When coffee bacterial leaf spot disease develops, all parts of the leaf can be infected. At the beginning of the disease, dark green water-stained spots appear on the diseased part and then expand into irregular-shaped spots with a dark brown center. Light brown or dark and light brown interspersed with the rest, the edges of the spots are irregular and slightly corrugated with faint water-stained marks, with a yellow halo at the periphery. Under wet conditions, the back of the spots exudes pus.

Based on the impact of coffee diseases on coffee yield and quality, Lifeasible continues to propose effective solutions for coffee disease prevention and control. It is committed to promoting the sustainable development of the coffee industry. We provide advanced biotechnology to provide effective technical support for coffee disease prevention and control, which is significant to the steady development of the coffee industry.

Damage of bacterial leaf spot disease

Coffee bacterial leaf spot disease is common in subtropical coffee growing areas. It mainly affects young coffee trees, especially those hit by strong typhoons, and often causes leaf drops, dead branches, and young fruit necrosis. It mainly affects the leaves, branches, and young fruits of medium-grain and small-grain coffee—specific manifestations of the following damage.

• Affect the growth of coffee trees
• Cause coffee yield reduction
• Decrease the quality of coffee fruit
• Affect the commercial value of coffee

Isolation and identification of bacterial leaf spot pathogens for selection and breeding of coffee varieties resistant to bacterial leaf spot

We combined the morphology of the pathogen and the ITS sequencing of the pathogenic bacteria to identify the bacterial leaf spot pathogen of coffee. We also identified the taxonomic status of the bacterial leaf spot pathogen of coffee as a Pseudomonas syringae coffee pathogenic variant. ITS identification is a method of DNA sequencing of ITS sequences and comparing the sequenced ITS sequences with known fungal ITS sequences. This is done to obtain information about the fungal species to be tested. We have used ITS sequencing to identify various plant pathogens and have achieved many research results.

We effectively identified the causal agent of coffee bacterial leaf spot disease by observing colony morphology, determining pathogenicity, and analyzing ITS sequences. We applied the results of this study to the screening of coffee varieties resistant to bacterial leaf spots. We further applied them to selecting and breeding coffee varieties resistant to bacterial leaf spots.

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Lifeasible offers a rapid and systematic method for identifying bacterial leaf spots in coffee. This method provides a solid foundation for research into the biology of coffee pathogens and the pathogenesis of the disease. In addition, our technical service can help select and breed disease-resistant coffee varieties to promote the sustainable development of the coffee industry and create significant economic value. If you are interested in us, please feel free to contact us.

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For research use only, not intended for any clinical use.

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