The diseases caused by plant viruses seriously affect the growth and development of plants and reduce the yield and quality of crops. Because there are many kinds of viruses and the harm mechanism is complex, plant detoxification has always been the focus of plant virology and plant breeding research. Lifeasible provides detoxification services for a variety of plant samples to customers around the world. The results of this service will help you research plant pathology, plant immunology, and plant culture and breeding.

What We Offer

• Genetic engineering detoxification

Through the foreign gene transformation vector constructed in vitro, a special structure of RNA is formed after transcription to effectively induce gene silencing, to resist the invasion and reproduction of the virus. A variety of resistance genes can be selected to control virus or virus compound infection.

• Detoxification method of stem tip tissue culture

The plant virus is unevenly distributed in the plant tissue and migrates with the vascular system in the plant, but there is no vascular bundle system in the stem tip meristem, and the cell division speed is fast, so there is almost no virus at the stem tip growth point, and the plant meristem contains high levels of endogenous hormones, which may inhibit virus replication.

• Detoxification method of heat treatment combined with stem tip culture

The protein is denatured by high temperature so that the virus is passivated, and finally, the virus-free plant is obtained.

• Detoxification method of cryopreservation combined with stem tip culture

The apical cell vacuole containing virus contains a lot of water, which is easy to form crystal to death in the process of cryopreservation (below -80℃), while the meristem with fast proliferation has dense cytoplasm and less water, so it is not easy to die in the process of cryopreservation. Virus-free plants were finally obtained.

• Chemical detoxification

According to the characteristics of the varieties, some antiviral agents were selected and added to the culture medium to remove the virus, which could significantly improve the probability of virus-free plants.

• Callus culture and detoxification

The callus was induced by selecting suitable explants, and then the callus was redifferentiated to form a complete regenerated plant. The replication rate of the virus in the callus is slower than that of the cells, or it may be because some of the cells acquire antiviral ability through mutation.

• Detoxification method of anther culture

The detoxification rate of anther culture is higher and the production cost of virus-free seedlings is lower. However, the rate of induced differentiation was lower and the culture cycle was longer.

Sample Requirement

• Seed material, >20 grains
• Seedling material, >6 plants
• Infected tissue material, >25 g

*The experimental samples will not be returned, please back them up by yourself. Before sampling, it is necessary to set up the control group and the experimental group, and each group is suggested to repeat more than 3 times.

*Quick-frozen preservation with liquid nitrogen can minimize the leakage time of plant samples at room temperature.

*There is no restriction on plant varieties. For varieties with special requirements or rare varieties, please contact our staff for more information.

We Do Better

Lifeasible is a leading integrated plant technology company, we not only have rich experience in the detection and classification of plant viruses, but also have been committed to solving various problems in plant detoxification research, and working with global researchers to find breakthroughs in plant detoxification research.

Reference

1. Zhibo Z., et al. "In vitro therapies for virus elimination of potato-valuable germplasm in Norway." Scientia Horticulturae, 2019, 249:7-14.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

For research use only, not intended for any clinical use.

Related Services

• Detection and Classification of Plant Viruses