Plant-pathogen interaction is a multifaceted process mediated by the pathogen- and plant-derived molecules, which mainly include proteins, sugars, and lipopolysaccharides. The use of non-destructive imaging methods holds great promise for early, efficient, and objective detection of plant responses to various stresses. Imaging is well suited to visualize heterogeneity in plant response to stress; localized responses can be revealed against a background of unaffected plant tissue.

At Lifeasible, we specialize in providing cutting-edge services for visualizing plant-pathogen interactions using various imaging techniques, including red light, thermal, and chlorophyll-fluorescence imaging. Our services enable researchers to gain valuable insights into plant-pathogen interactions and enhance disease management strategies.

Red Light Imaging for Visualizing Plant-Pathogen Interaction

• We use red light imaging to visualize plant-pathogen interactions which employs red light-emitting diodes (LEDs) to illuminate the plants while specialized cameras capture the emitted light.
• We analyze the captured images to quantify the extent of pathogen colonization and determine the spatial distribution of pathogens on plant surfaces.
• We use this technology for studying foliar diseases, where pathogens primarily target the leaves of host plants. It enables the visualization of pathogen spore deposition, colonization, and spread.

Thermal Imaging for Visualizing Plant-Pathogen Interaction

Fig. 1 TMV-lesion development in attached tobacco leaves visualized by color reflectance, thermal and chlorophyll fluorescence time-lapse imaging. (Chaerle L, et al., 2004)

• We use thermal imaging as another valuable technique for visualizing plant-pathogen interactions. This method utilizes infrared cameras to measure the thermal radiation emitted by objects, including plants and pathogens.
• Our thermal imaging has several advantages for studying plant-pathogen interactions. It allows for non-invasive and real-time monitoring of plant health and disease progression. By identifying temperature anomalies on plant surfaces, researchers can pinpoint areas of pathogen colonization. Furthermore, thermal imaging can also provide insights into the physiological responses of plants to pathogen infection, such as alterations in heat dissipation and transpiration rates.

Chlorophyll-Fluorescence Imaging for Visualizing Plant-Pathogen Interaction

• We offer chlorophyll-fluorescence imaging for research on plant-pathogen interaction, measuring the fluorescence emission from chlorophyll molecules in response to light excitation. By analyzing the fluorescence emitted by infected plants, researchers can gain insights into the physiological responses of plants to pathogen attacks.
• We can quantify changes in fluorescence parameters, such as the maximum quantum yield of photosystem II (Fv/Fm) and the efficiency of electron transport; researchers can assess the impact of pathogen infection on plant photosynthetic performance and overall plant health. Therefore, this technique allows for non-destructive and high-throughput analysis of plant responses to infection.

At Lifeasible, our team of experienced scientists and researchers ensures that our services are tailored to meet the specific needs of our clients. If you are interested in our services or have questions, please feel free to contact us or make an online inquiry.

Reference

1. Chaerle L, et al. (2004). "Thermal and chlorophyll-fluorescence imaging distinguish plant-pathogen interactions at an early stage." Plant Cell Physiol. 45 (7), 887-96.

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