Long non-coding RNAs (lncRNAs) are now recognized as essential players in gene regulation, and their potential regulatory roles are being studied comprehensively in eukaryotes. Efforts are being made using computational tools and high-throughput sequencing technologies, such as the lncRNA microarray technique, RNA-sequencing (RNA-seq), etc. Microarrays are widely used for gene expression analysis and preferred over RNA Seq for expression profiling of known transcripts because of their low cost and simplicity of data analysis. Based on probe design, microarrays are subdivided into non-tiling and tiling arrays.

Lifeasible provides a one-stop technical service for the whole process of plant lncRNA microarray, completes all experimental operations for our customers, and provides complete experimental reports. Meanwhile, we provide in-depth data mining services according to our customers' research needs. Our platform is equipped with cutting-edge facilities and professional experts to support research.

Tiling Arrays

• LncRNAs are usually expressed and function in low abundance, hidden among other classes of abundant RNA. Using RNA-seq for lncRNA expression studies has many limitations, and lncRNA arrays are a more sensitive and better alternative.
• For tiling arrays, the probes are designed in an overlapping fashion and cover the whole genome or the entire length of the desired portion of the genome. Tiling arrays can work for both annotated and unannotated regions of the genome; hence, this method can identify novel transcripts. In tiling arrays, neighboring probes on a plate hybridize to overlapping regions of the genome, thereby covering the entire region genomic region of interest.
• We provide plant lncRNA identification services to our clients using tiling microarrays in Arabidopsis, wheat, and other plant species. Our complete experimental reports and output data files are delivered to clients according to their research needs.
• We provide comprehensive experimental reports, including microarray scans, experimental method reports, RNA quality control reports, microarray data result reports (list of differentially expressed lncRNAs and genes), and others.

General Workflow

• Total RNA extraction from the sample.
• RNA quality assessment.
• cDNA synthesis and labeling.
• Quality testing of labeling efficiency. Detects fluorescent labeling efficiency to ensure the reliability of subsequent microarray experimental results.
• Chip hybridization. The labeled probes are hybridized with high-density microarrays under standard conditions.
• Image acquisition and data analysis. The fluorescence intensity of the chip is scanned using a chip scanner, and the experimental results are converted into digital-type data and saved. After that, the corresponding software is used to analyze and calculate the raw data.

Lifeasible provides cost-effective, high-quality, and hassle-free services to our customers worldwide. We provide our clients with direct access to our experts and prompt responses to their questions. If you are interested in our services or have questions, please contact us or make an online inquiry.

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For research use only, not intended for any clinical use.

Related Services

• Plant lncRNA Sequencing
• Bioinformatics Analysis