CRISPR/Cas9 genome editing technology has become a revolutionary tool in the life sciences, providing efficient, rapid, and inexpensive genetic manipulation tools for basic botanical research and crop genetic improvement. Genetic manipulations such as precise knock-out and knock-in can be achieved using the CRISPR/Cas9 system, which can also be used to target activation or repression of gene expression. While CRISPR/Cas9 is widely used for genome editing, its editing capability, efficiency, and precision are constantly being improved and refined. In particular, the discovery of the CRISPR/Cpf1 system and the creation of single-base editing technology are gradually making the CRISPR system an ideal technological platform for genetic engineering. In addition, crop varieties improved by CRISPR/Cas9 technology have emerged, which will certainly promote the application and development of precision genome editing technology in crop genetic improvement.

What We Offer

Lifeasible provides targeted scientific solutions for different clients, and the general process includes gRNA design, CRISPR/Cas9 vector construction, gRNA target activity detection, genetic transformation, identification, and final transformant acquisition. Lifeasible provides a complete set of plant gene-editing technology services, plant genetic transformation services, plant overexpression transgenic vector construction and transgenic services, plant functional complementation transgenic vector construction, and transgenic services, plant gene interference (RNAi) vector construction and transgenic services, etc. The services we provide are simple, efficient, multi-target editing, and diverse in form, and can be used for plant trait improvement, functional genomics, and transgenic plant acquisition.

We Do Better

• We provide a complete set of solutions from sgRNA design, Cas9 codon optimization, sgRNA, and Cas9 synthesis, and construction and activity detection, aiming to provide stable and efficient plant gene editing and genetic transformation services.
• Our exclusive sgRNA design and codon optimization platform can provide efficient and accurate multi-species sequence design solutions and optimize Cas9 protein sequences to ensure efficient translation in plant cells.
• We have made targeted improvements to the CRISPR/Cas9 system, which is more suitable for plant gene editing with a high editing rate and low off-target effect, providing cost-effective services to our customers.

Reference

1. Sedeek K. E. M., et al., “Plant Genome Engineering for Targeted Improvement of Crop Traits.” Frontiers in Plant Science, 2019,10:114.

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Related Services

• Plant Genetic Transformation
• Plant Gene Family Analysis