Artificial chromosomes usually refer to artificially constructed vector systems containing the basic functional units of natural chromosomes, including yeast artificial chromosomes (YAC), bacterial artificial chromosomes (BAC), human artificial chromosomes (HAC), and plant artificial chromosomes (PAC). PACs may serve as the main carriers of the next generation of transgenes and have great potential in modifying transgenic crops or producing antibody proteins for pharmaceutical use. In the field of epigenetics, Lifeasible is also actively promoting the PAC system, aiming to apply it to the regulation of gene expression and cross-disciplinary research.

An artificial chromosome usually has three parts: a mitosome, a gene cassette, and a chromosome terminal, the challenging part being the mitosome. We can use telomere truncation to construct PACs and transfer target genes to PACs through site-specific recombination systems.

• Establishment of a technical system for identifying plant chromosome components such as the mitosome, telomere, and origin of replication.
• Identify critical components of plant artificial chromosomes.
• Identify the contribution of transposons and repetitive sequences to chromosome components.
• Establish a yeast-based system for synthesizing large segments of plant artificial chromosomes.

We can lap segment lapping of PAC with the help of the chromosome stepping technique. In addition, we also obtain critical regulatory genes in plants based on known genes or molecular markers in successive steps, which can be used to study the regulation of the expression of structural genes.

• Step checking acquires non-conserved regions of genes in new species, thus obtaining complete gene sequences.
• Identification of insertion sites of T-DNA or transposons, identification of exogenous genes resulting from transgenic techniques such as the gene gun transgenic method, etc.
• Gap filling in chromosome sequencing efforts to obtain the complete genome sequence.

We construct PACs that carry large fragments of DNA ranging from tens to thousands of kilobases in length. They can be used to construct genomic libraries of higher organisms and for whole genome sequencing. By combining the construction of genomic libraries with molecular breeding, we can select beneficial genes from libraries for functional analysis and transform plants simultaneously.

Lifeasible's genome design and construction services in plants will enable the synthesis of genomes in multicellular organisms with complex genome structures and pervasive and fine-grained epigenetic regulation. They will provide a platform for rapid evolution, remodeling of metabolic pathways, and enhanced epigenetic research. Please feel free to contact us to develop more PACs available at the intersection of epigenetics.

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For research use only, not intended for any clinical use.

Related Services

• Analysis of Transposable Elements
• Gene Expression Assays
• Analysis of Gene Imprinting
• Epigenome Mapping