Chromatin is divided into euchromatin and heterochromatin, whereas euchromatin is in a loosely open state, allowing trans-acting factors to bind cis-regulatory elements, which regulate gene expression. Open chromatin is transposase-sensitive, and transposases can insert randomly into unprotected DNA sequences (similar to DNase), but transposases do not cleave DNA like restriction endonucleases. Assay for transposase-accessible chromatin with high throughput sequencing (ATAC-seq) utilizes a modified Tn5 transposase to cleave open DNA regions while ligating sequencing junctions. Lifeasible offers ATAC-seq technology services that combine high-throughput sequencing and biosignature analysis to study open regions of chromatin on a genome-wide scale systematically.

We utilized the efficient transposition activity of Tn5 super-sensitive transposase to interrupt the DNA in open chromatin regions while adding sequencing junctions to achieve efficient library construction. We use ATAC-seq technology to study open chromatin regions, transcription factor binding sites, and nucleosome occupancy simultaneously. It requires a low starting volume of samples, a small amount of sequencing data, and high reproducibility, which can avoid the disadvantages of DNase-seq and MNase-seq, such as high starting volume, complicated operation, and poor reproducibility. We can provide data evaluation and quality control, comparison with the reference genome, Peaks analysis, and other analyses. We are equipped with special technical support, which can provide you with the whole technical communication process.

Tn5 transposase binds easily to open chromatin. Using the modified Tn5 transposase, the transposable DNA is designed as a junction sequence, which can introduce the sequencing junction directly into the open chromatin and sequence the fragments. Because Tn5 cannot cut, it can capture the entire open region directly, so we can widely apply ATAC-seq to open chromosome sequencing.

• Lower requirements for sample size.
• Capture the entire open region.
• Libraries of 10,000-20,000 cells are usually completed within 2 hours.
• Combining ATAC-seq with other sequencing tools (e.g., ChIP-seq or RNA-seq) for multi-omics analysis is possible.

ATAC-seq is well able to identify accessible chromatin, is simple, fast to implement, and can be applied to limited tissue samples under realistic conditions. The ATAC-seq technical service provided by Lifeasible has been widely used in the study of transcriptional regulatory mechanisms, transcription factor binding analysis, nucleosome localization, distribution of active regulatory elements, etc.. It has a broad application prospect in the field of epigenetic mechanism research. Please feel free to contact us for the information you need.

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For research use only, not intended for any clinical use.

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