Among the known coffee varieties, Arabica and Robusta enjoy the highest economic value. There is no way to determine whether a ground coffee is the expensive Arabica coffee or the inexpensive Robusta coffee just from the appearance of the ground coffee. A viable method of identification is to analyze the marker 16-O-methylcafestol (16-OMC) in it.

Lifeasible is a biological company focused on plant research, and we offer a comprehensive range of plant science research services. We utilize an advanced technology platform to provide an effective solution for determining coffee alcohol methyl ester in roasted coffee.

Application of the determination of 16-OMC in roasted coffee

Due to the obvious differences in the appearance of different varieties of coffee beans, consumers can easily distinguish if there is a mix of raw and roasted beans. However, the same cannot be said for coffee powder and instant coffee. Since these products are generally heavily roasted, the flavor differences between varieties are more difficult to distinguish. Therefore, it is easy for some sellers to mix low-priced beans to determine the content of 16-OMC in roasted coffee, to ensure the authenticity of the coffee market, to avoid the "coffee mixed beans" situation, to truly protect the rights and interests of consumers, to combat international food fraud to provide effective technical support.

We offer various effective solutions for the determination of 16-OMC in roasted coffee

• HPLC analysis method

Using the HPLC analytical method, we have been widely used to determine the 16-OMC content in roasted coffee. Using this technique, we can indicate the 16-OMC content in roasted coffee by chromatogram. For example, since 16-OMC is only present in Robusta, it is completely absent in Arabica. Thus, using the HPLC analysis method, we can clearly identify the authenticity of roasted coffee varieties using specialized detection techniques.

• MS identification method

Since the UV spectrum of 16-OMC is not characteristic and may overlap peaks, such as coffee-like lipids that are not fully hydrolyzed, simple UV detectors or diode array detectors are not well suited, resulting in false Robusta identifications or higher levels of 16-OMC than those present in the sample. We used an HPLC-MS system to identify a more reliable determination of 16-OMC in roasted coffee.

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• Full coverage of testing types to meet the needs of various indicators testing.
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Lifeasible has many years of service in the field of plant research. Providing our clients with high-quality project results has always been our standard of service. If you are interested in us, please feel free to contact us.

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