Alternative splicing (AS) is a form of transcriptional regulation that enables the production of different mRNA isoforms from a single gene, which may then be translated to produce different proteins. Plant lncRNAs play a role in transcriptional regulation and are regulators of mRNA precursor splicing. They mainly regulate AS through interactions with specific SFs, by regulating chromatin remodeling that fine-tunes the splicing of specific targets, and via the formation of lncRNA-pre-mRNA duplexes.

Fig. 1 Long noncoding RNAs modulating alternative splicing. (Fonouni-Farde C, et al., 2021)

Lifeasible provides analysis service of plant lncRNA in the regulation of alternative splicing to help our customers worldwide in plant science research. Our platform is equipped with cutting-edge facilities and professional experts to support research. Here, we provide various services according to customers' demands.

Interacting with Splicing Factors

• A splicing factor (SF) is a protein involved in removing introns from strings of messenger RNA so that the exons can bind together. Based on the interaction between plant lncRNA and SFs, AS plays crucial functions in controlling gene expression, boosting the protein-coding capacity, and contributing to developmental plasticity in plants.
• We provide analysis services for plant lncRNA that interact with splicing factors. In Arabidopsis, the lncRNA ALTERNATIVE SPLICING COMPETITOR (ASCO) interacts in vivo with the plant-specific SFs. Our research techniques include generating transgenic plants with gene overexpression and knockout, RNA immunoprecipitation (RIP)-Seq approach, chromatin isolation by RNA purification (ChIRP), mass spectrometry, and others. Our technical team will make a suitable solution according to the purpose of our client's research.

Regulating Splicing Through Chromatin Remodeling

• Chromatin remodeling is the rearrangement of chromatin from a condensed state to a transcriptionally accessible state, allowing transcription factors or other DNA binding proteins to access DNA and control gene expression.
• We help our clients analyze the role of plant lncRNA in regulating splicing through chromatin remodeling. Circular non-coding RNAs (circRNAs) expressed from distant loci may increase the splicing efficiency of their cognate exon-skipped messenger RNAs, and chromatin conformation and R-loop formation are critical modulators of splicing patterns.

Regulating Splicing Through LncRNA-RNA Duplexes

We also help our customers analyze the plant lncRNA-RNA duplexes in regulating AS. The loci where antisense transcripts overlap with sense transcript introns mostly show AS and/or variation of polyadenylation. If sense and antisense are expressed in the same subcellular compartment, most of them form extended double-stranded RNA duplexes, and they may lead to gene silencing and indirect AS regulation.

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Reference

1. Fonouni-Farde C, et al. (2021). "Plant Long Noncoding RNAs: New Players in the Field of Post-Transcriptional Regulations." Noncoding RNA. 7 (1), 12.

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