Yeast Screening and Identification

Yeasts are a group of eukaryotic microorganisms that are predominantly single-celled and reproduce by germination. Most yeasts are saprophytic and live-in environments with high sugar content and acidic (pH 4.5-6), such as fruits, vegetables, nectar, and plant leaves, especially in the soil of orchards, vineyards, and vegetable gardens. A few are parasitic and can cause diseases in humans, animals, and plants.

Yeast screening and identification

Yeast as a host bacterium to build genetic engineering bacteria is gaining more and more attention because yeast growth and reproduction are fast, the culture medium and culture conditions are simple, more importantly, yeast production capacity, with the ability to secrete and glycosylate exogenous protein, therefore, it plays a huge potential in the production of genetic engineering products, and with the increasing demand for health care, for yeast-related drugs and With the increasing demand for healthcare, the development and utilization of yeast-related drugs and health food products is gaining more and more attention from researchers. There is no doubt that the screening and identification of yeast strains will be the most important cornerstone of the industry chain.

What We Offer

Traditional yeast classification and identification methods

  • The classification of yeast relies mainly on morphological description and physiological and biochemical characteristics. Since yeasts exist in the form of single cells, the description of their morphological characteristics is very limited, so in determining the classification of their species we mainly rely on physiological characteristics such as the assimilation ability of different carbon and nitrogen sources, the effect of different temperatures on their growth and the variability of dependence on exogenous vitamins. However, all these characteristics reflect variability at the phenotypic level and it is difficult to determine their interspecific relationships. Although certain carbon sources are not identical in type and structure, they may share the same or similar metabolic pathways. They reflect limited genetic information. Therefore, Lifeasible does not recommend clients to choose only traditional taxonomic identification services.

Molecular biology identification methods

  • Because of the limitations of traditional taxonomic identification so Lifeasible introduced molecular biology identification techniques. Since the molecular biology method is mainly based on nucleic acids, the genotype is analyzed instead of the phenotype, thus reflecting the genetic nature. This feature makes up for the shortcomings and deficiencies of morphological and physiological and biochemical identification methods. The method is stable, easy to determine homology, and convenient for computer analysis, so it has a faster identification speed and more reliable identification results.

Analysis content

  • DNA G+C molar percentage content analysis
  • Pulsed Field Gel Electrophoresis (PFGE) analysis
  • Random amplification of polymorphic DNA (RAPD)
  • DNA restriction enzyme fragment length polymorphism (RFLP)
  • 26S rDNA D1/D2 region sequence analysis
  • High-throughput sequencing

We Do Better

As a growing and improving phytotechnology company, Lifeasible not only provides services based on traditional technologies, but we have become proficient in applying high-throughput sequencing technologies to microbial identification analysis, providing important basic data for your research projects through the analysis of yeast genomes.

The services provided by Lifeasible cover all aspects of plant research, please contact us to find out how we can help you achieve the next research breakthrough.

Contact

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