More than 100 modifications exist in RNA, and RNA methylation modifications account for about 60% or more of all RNA modifications. Some non-coding RNAs, such as lncRNAs, tRNAs, rRNAs, and shear body RNAs, also have substantial base modification activities before and after transcription. In eukaryotes, Cap at the 5' end and ployA at the 3' end play critical roles in transcriptional regulation. At the same time, internal modifications of mRNAs are used to maintain the stability of mRNAs. mRNAs are most commonly modified internally, including m6A, m1A, and m5C. Lifeasible provides machine learning-based RNA methylation modification site prediction and m6A methylation detection services to help you explore deeper information about RNA methylation modification in epigenetics.

RNA methylation modification site prediction

Deep learning, developed from artificial neural networks, has algorithmic models with stronger generalization ability and more accurate fitting results for unknown datasets, and deep learning algorithms trained on large numbers of datasets can improve the prediction accuracy of RNA methylation modification sites. We are actively expanding the dataset size, establishing clear dataset metrics, increasing the number of species, and using deep learning algorithms to improve RNA methylation site prediction accuracy.

Plant species available for m6A methylation detection

MeRIP-seq

MeRIP-seq (m6A-seq) detects m6A modifications on a transcriptome-wide scale. The technology uses an m6A antibody to enrich methylated RNA fragments, which is then combined with high-throughput sequencing to detect m6A modifications on a transcriptome-wide scale.

MeRIP-qPCR

qPCR validation of differential m6A genes/transcripts is essential in epitranscriptomics studies. meRIP-qPCR quantitative assay is a technique that combines RNA immunoprecipitation (RIP) with fluorescence quantitative PCR, which is used to enrich m6A methylation-modified RNA in total RNA by a specific antibody, and with the help of specific primers to achieve the quantification of m6A-modified mRNA, lncRNA or circRNA with the help of specific primers. We provide MeRIP-qPCR service corresponding to MeRIP-seq, which guarantees the optimal experimental process and the best technical solution and can be applied to the m6A-modified genes identified by MeRIP-seq (or a known target gene) in the differences of m6A modification levels between groups of samples.

Total RNA / mRNA m6A quantification (Dot-blot assay)

Dot-blot is an antibody-based modification detection method that can easily and quickly determine the number of overall m6A modifications in RNA samples. Compared to mass spectrometry, the method is less expensive. Meanwhile, to achieve m6A detection and quantification of individual sites, we will use the T3 ligase method and the MazF nucleic acid endonuclease method in our experiments, which makes our analyses low-cost and high sensitivity.

RNA is an important carrier embodying cellular life activities, decoding and recoding genetic information through transcription and post-transcriptional regulation, and participating in plant growth and metabolism. Lifeasible has promoted RNA methylation modification analysis in several plant species, which can help you with studies on fruit ripening regulation, virus infection, increasing plant yield and biomass, abiotic resistance, and more. Please feel free to contact us for species-specific analysis protocols.

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For research or industrial use.

Related Services

• Restriction Endonuclease-Based DNA Methylation Detection
• Bisulfite-Based DNA Methylation Detection
• Immunoprecipitation of Methylated DNA
• SMRT Sequencing for Direct Detection of DNA Methylation
• Single Molecule Nanopore Dependent Methylation Sequencing
• RNA-Directed DNA Methylation
• DNA Hydroxymethylation