Crossbreeding is often accompanied by a long segregation process, numerous types of recombination, longer breeding time, and greater workload, and can result in the loss of recessive genes, so it is difficult to select for excellent overall traits. As an important means of haploid breeding, anther culture technology can be used to obtain haploid plants through isolated culture and then double them, which can obtain pure and stable lines in a shorter period of time and enable the expression of some recessive gene-controlled traits, i.e., shorten the breeding cycle and at the same time enrich the genetic base of the selected good lines, which is an important way of germplasm innovation and new variety selection and breeding. And, anther culture has the advantages of accelerating the purity of hybrid progeny, shortening the breeding cycle, and improving selection efficiency.

What We Offer

Lifeasible uses anther culture to induce haploid plants and then doubles the haploids to obtain pure lines that meet the breeding objectives, and we also combine other breeding methods depending on the material, which greatly shortens the breeding period and improves breeding efficiency.

In anther culture, the genotype and growing environment of the test material, the dedifferentiation medium and additional components, culture conditions, anther development period, pretreatment, inoculation density, and doubling of haploid plants are all important factors affecting the culture effect. Lifeasible has a strict control program for each of these factors.

• Medium types: MS, NTH, N6, B5, NH, C, R, CP, and other solid media.
• Growth regulators: NAA, IAA, 2,4-D, KT, ZT, 6-BA.
• Other components: activated carbon, AgNO₃, vitamin C, coconut milk, nucleotides, and hydrolyzed casein.
• Ploidy identification: morphological observation method, chromosome counting method, flow cytometry assay, stomatal guard cell chloroplast counting method.

We Do Better

Lifeasible, a leading plant technology company, has extensive experience in plant culture. Our team has obtained haploid plants in anther culture on wheat, rice, corn, tobacco, eggplant, rape, sugar beet, grape, pepper, and other crops, and has extensive project experience to deliver customer projects quickly and to a high standard.

Reference

1. Germanà M. A., “Anther culture for haploid and doubled haploid production.” Plant Cell Tiss Organ Cult, 2011, 104: 283-300.

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