Flowering is a necessary life process in the transition from vegetative to generative growth in higher plants. From a developmental perspective, flower formation starts with the triggering of specific pathways in the founder cells of lateral organs (i.e., leaves initially) so that they develop into flowers instead. This developmental switch is called the floral transition. Plant flowering pathways include the vernalization-promoting, photoperiod-promoting, autonomous flowering, and gibberellin pathways. Some lncRNAs are involved in the vernalization pathway and photoperiod pathway in plants.

Lifeasible provides analysis services for plant lncRNA in regulating floral transition via vernalization and photoperiod pathways to help our customers worldwide in plant science research. Our platform is equipped with cutting-edge facilities and professional experts to support research. Here, we provide various services according to customers' demands.

Plant lncRNA Involved in Vernalization

• Vernalization is the induction of the flowering process of the plant by exposure to long periods of cold winter or such conditions. Once this process takes place, plants develop the capability of flowering. Expression of the flowering locus C (FLC) is an essential factor affecting the flowering transition in plants.

• We provide analysis services for plant lncRNA COOLAIR involved in vernalization to regulate floral transition. It is a class of antisense transcripts at the 3ʹ end of the FLC gene, which removes the activation type group H3K27me3 marker from the FLC gene by attracting RNA binding protein flowering control locus A (FCA), thus causing the silencing of FLC gene.
• We help our clients analyze the role of plant lncRNA COLDAIR in vernalization for regulating floral transition. It is transcribed from the second intron of the FLC gene, and the interaction with the RNA-binding protein FCA triggers the enrichment of the PRC2 subunit curly leaf (CLF) at the FLC gene locus with H3K27me3, which in turn suppresses the expression of the FLC gene.
• We also offer analysis services for plant lncRNA COLDWRAP involved in vernalization to regulate floral transition. It is an FLC gene promoter-associated lncRNA that also interacts with CLF to form intragenic chromatin loops and repress FLC genes. Our services involve multiple species and can explore other lncRNAs according to the client's needs.

Plant lncRNA Involved in Photoperiod

• The photoperiod, which is the length of the light period in the diurnal cycle of 24 h, is an essential environmental signal. Plants have evolved sensitive mechanisms to measure the length of the photoperiod.

• We provide analysis services for plant lncRNA involved in photoperiod to regulate floral transition, mainly FLORE. It promotes flowering by suppressing the expression levels of the cycling of factors (CDFs) transcription factor genes CDF5, CDF1, and CDF3, thereby increasing the expression of FT genes.

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