Microbial genome de novo sequencing is a sequencing technology based on second- or third-generation high-throughput sequencing platforms and bioinformatic analysis technologies to examine the genomes of microorganisms and thus obtain information on the coding and variation of bacterial genomes. Lifeasible combines the long-read length and high accuracy of the platform to efficiently perform the de novo assembly of microbial genomes using third- and second-generation sequencing data. de novo assembly of microbial genomes. This type of sequencing allows whole genome splicing and assembly without the need for gene sequence information of the species. In obtaining the whole genome sequence map, not only can the evolutionary history of the unknown species be analyzed, but more importantly, it can provide a convenient and efficient platform for the subsequent research of the species, making gene mining and functional verification more convenient and faster, and also providing a reference database for more profound histological research.

What We Offer

• Bacterial de novo sequencing

Bacterial genome sequencing is mainly performed for species without reference sequences, and sample genomes are sequenced and assembled from scratch. Depending on the characteristics of the species itself and the sequencing depth, the final assembly results will vary somewhat. For bacterial samples, up to one complete sequence can be assembled. Based on the assembly results, the genes contained in the bacterial genome can be predicted, and the functional annotation information of the genes can be obtained by functional database comparison. It has a wide range of applications in the pathogenicity of plant pathogens, prevention, and treatment of diseases.

• Fungal de novo sequencing

Fungal de novo sequencing, a sequencing analysis technique that allows the analysis of a fungus without relying on any reference sequence information. Sequence splicing by bioinformatics can predict the genes contained in the fungal genome, thus obtaining the genome sequence map of the species and performing a series of data analyses such as functional annotation.

We Do Better

• Advanced sequencing platform. We use long-length high-throughput sequencing platforms, averaging about 10 k, to provide high-quality sequencing data.
• Experienced and fast delivery. We have completed tens of thousands of bacterial de novo projects and have a well-developed analysis process with one-click delivery, greatly reducing the project cycle time.
• Professional and comprehensive services. We have a standardized experimental operation team to provide professional protocol design, bacterial and fungal genome analysis, and subsequent interpretation of test reports.
• Personalized services. We provide comprehensive testing services and professional experimental guidance and customized advanced analysis to meet our customers' individual analysis needs.

Reference

1. Knief C., et al., "Analysis of plant microbe interactions in the era of next generation sequencing technologies." Frontiers in Plant Science, 2014, 5: 216.

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For research use only, not intended for any clinical use.

Related Services

• Microbial Detection and Contamination Analysis
• Microbial Diversity Sequencing Analysis
• Metagenomics Sequencing Analysis
• Microbial Genome Resequencing