RNA interference (RNAi), known as RNA silencing, is a nucleotide sequence-specific epigenetic effect that leads to RNA degradation, protein translation inhibition, DNA methylation, and heterochromatin formation. Lifeasible provides customers with RNAi technology, which uses artificial methods to introduce silencing inducers into the host to degrade the transcripts of target genes.

• Introduce in vitro synthesized siRNAs, or dsRNAs containing their sequences, into host cells using methods such as injection.
• The precursor sequence of miRNA associated with the target gene or invert repeat RNA (IR-RNA) is expressed in the host to induce degradation of the target gene mRNA. The method method is stable and easy to implement.
• Virus-induced gene silencing (VIGS) is induced by plant virus infestation. We use the principle of VIGS to recombine target genes with viral vectors, which can also induce silencing of target genes when used to infect plants. This is usually achieved by direct injection of transcripts in vitro, gene guns, etc. We can silence multi-copy genes and multi-gene families.

RNAi-mediated resistance to plant viruses

RNAi-based tools are used in various organisms where complementary small RNAs specifically degrade target genes and induce antiviral resistance through the sequence of the target RNA. In plants, highly specific antiviral RNAi tools include artificial microRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs). Syn-tasiRNA has emerged as a promising antiviral tool, and we can achieve multi-targeting of viral RNAs by simultaneously expressing several syn-tasiRNAs derived from a single precursor.

hpRNAi technology

We utilize the structure of an intron between the positive and negative arms to improve the efficiency of gene silencing. Long fragments of hairpin RNA (hpRNA) expressed in plants form siRNAs in response to the Dicer enzyme, which induces degradation of the target RNA.

Argonaute (AGO) protein

AGOs are required for RNAi and are involved in gene silencing through target mRNA cleavage, translational repression, or chromatin modification. We provide services to identify the genes encoding AGO proteins in different plant species. We also offer a functional study of the AGO if you need it. Please feel free to submit your research needs for evaluation by our staff.

The RNAi pathway can repress target genes at the transcriptional level by episodic modification of chromosomes through histone or DNA methylation in the nucleus. In addition to hpRNAi and VIGS, Lifeasible also offers RNA silencing technologies such as co-repression and antisense repression, which can be used to varying degrees to meet your needs for suppressing gene expression. Please feel free to contact us for a more suitable solution for you.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

For research use only, not intended for any clinical use.

Related Services

• Small RNA Regulation Analysis
• Small RNA Library Analysis
• Non-Coding RNA Gene Identification
• Theoretical Predictions of Non-Coding RNA Genes
• MicroRNA Detection
• RNA Modification Detection