In contrast to gain-of-function experiments, loss-of-function experiments, represented by RNA interference (RNAi), complement each other to illustrate the functional role of lncRNA genes in plants. Knockdown of lncRNAs is the specific degradation of homologous RNAs mediated by small interfering RNAs (siRNAs) and short hairpin RNAs (shRNAs), one of the most dominant and common techniques in lncRNA functional studies.

Lifeasible provides loss-of-function analyses of plant lncRNA, mediated by virus-induced gene silencing, small interfering RNA, and short hairpin RNA, to help our customers worldwide in plant science research. Our platform is equipped with cutting-edge facilities and professional experts to support research. Here, we provide various services according to customers' demands.

Loss-Of-Function of Plant lncRNA Mediated by VIGS

• Virus-induced gene silencing (VIGS) is an effective tool for plant gene function analysis. Over the last decade, VIGS has been successfully used as both a forward and reverse genetics technique for gene function analysis in various model plants and crop plants.

Fig. 1 VIGS molecular mechanism of action.

• We help our customers to perform VIGS-mediated loss of function of lncRNAs in a variety of plants, such as Arabidopsis, tobacco, barley, rice, maize, potato, etc. Our services include VIGS vector construction, recombinant VIGS vector transformation of E. coli and sequence identification, VIGS vector transformation of Agrobacterium, VIGS infestation of plant experiments, and phenotype identification.

Loss-Of-Function of Plant lncRNA Mediated by siRNA

• siRNA, also known as silencing RNA and short-interfering RNA, is a double-stranded RNA molecule that is non-coding and operates within the RNA interference pathway. In addition, siRNAs have very tight target specificity as they cleave the mRNA before translation.

Fig. 2 Schematic diagram of siRNA infestation.

• We help customers to achieve loss-of-function of plant lncRNA mediated by siRNA. Our services include designing and synthesizing siRNA based on lncRNA sequences, verifying the silencing efficiency of siRNA by qPCR and Western Blot, screening the optimal concentration of siRNA, and performing plant transfection and phenotype observation. In addition, we provide our clients with raw experimental data, methods, and results report sheets in a timely manner.

Loss-Of-Function of Plant lncRNA Mediated by shRNA

shRNA is a type of RNAi technology leveraged to study the function of unknown genes. According to the demand of customer research, we provide loss-of-function analysis service of plant lncRNA mediated by shRNA. Please contact our staff for specific details.

Lifeasible provides cost-effective, high-quality, and hassle-free services to our customers worldwide. We provide our clients with direct access to our experts and prompt responses to their questions. If you are interested in our services or have questions, please contact us or make an online inquiry.

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Related Services

• Plant lncRNA Gain-of-Function Analysis
• Functional Analysis of Plant lncRNA with CRISPR-Cas Technology
• Plant lncRNA Other Knockdown Analysis