Single-cell RNA sequencing (scRNA-seq) is the most widely used single-cell transcriptome technology for plants, unlocking tremendous potential in plant science. As an industry-leading plant technology company, Lifeasible provides droplet-based and plate-based scRNA-seq technology platforms to capture plant transcriptional changes at single-cell resolution.

Introduction to Plant scRNA-seq

Using sequencing technologies in plants to analyze genetic variation and metabolic regulation plays a and vital role in enhancing our understanding of plant developmental processes and responses to stimuli. However, traditional sequencing methods can only generate average cellular data and cannot analyze large numbers of cells, thus losing information on cellular heterogeneity. Advances in single-cell RNA sequencing (scRNA-seq) technology have enabled researchers to explore the complex details of gene expression at the single-cell level and obtain genomic or transcriptomic information that can reveal heterogeneity among cell populations. By analyzing individual cells within plant tissues, scRNA-seq has revolutionized our understanding of cellular heterogeneity, developmental processes, and responses to environmental cues. In summary, the opportunity to observe intercellular heterogeneity at the resolution allowed by high-throughput scRNA-Seq opens up new perspectives on a range of critical questions in plant biology.

Fig.1. Proposed workflow for single-cell RNA sequencing. (Bawa G, et al., 2022)

What We Offer

Lifeasible is a pioneer in plant biotechnology, offering a comprehensive service for plant single-cell RNA sequencing (scRNA-seq) that allows identifying individual cells at high resolution, discovering new cells, and understanding cellular uniqueness. With our state-of-the-art facilities and molecular biology expertise, we are committed to helping our clients unravel the complexity of plant cell populations and their gene expression profiles. Our services cover every step of the scRNA-seq workflow, ensuring accurate and reliable results.

(1) Cell Isolation and Sample Preparation

Lifeasible uses optimized protocols to isolate individual plant cells, ensuring their viability and integrity throughout the process. We have expertise in various cell isolation techniques, including enzymatic dissociation, fluorescence-activated cell sorting (FACS), and microfluidic-based methods. Our skilled technicians employ stringent quality control measures to ensure high-quality samples for downstream analysis.

(2) Library Preparation and Sequencing

Lifeasible uses cutting-edge library preparation solutions designed specifically for scRNA-seq. We use methods such as droplet-based and plate-based scRNA-seq technologies to accurately quantify gene expression at the single-cell level. Our state-of-the-art sequencing platforms generate high-throughput data with excellent depth and resolution, allowing for a comprehensive exploration of the transcriptome landscape within plant cells.

(3) Data Analysis and Interpretation

Lifeasible's expertise goes beyond data generation as we offer comprehensive bioinformatics analysis tailored to scRNA-seq data. Our skilled bioinformaticians utilize advanced algorithms and computational tools to process, analyze, and interpret the vast amounts of data generated by scRNA-seq experiments. We provide custom solutions for data visualization, differential gene expression analysis, clustering, and trajectory inference, enabling researchers to gain valuable insights into cellular heterogeneity and regulatory networks.

Our Plant scRNA-seq Platforms

(1) Droplet-based scRNA-seq Platform

• Direct sorting of individual cells using droplets provides indiscriminate access to thousands of cells in tissues.
• This can yield greater cell throughput and lower sequencing cost per cell.

(2) Plate-based scRNA-seq Platform

• Suitable for studying specific types or rare cells, such as germ cells.
• Usually combined with capillary mouth aspiration, laser microdissection, or flow cytometric fluorescence sorting techniques to sort individual cells.
• The advantage is high capture efficiency, but the sequencing cost is high due to its low cell throughput.

Lifeasible offers comprehensive services for plant scRNA-seq, from cell isolation and sample preparation to library preparation, sequencing, and data analysis. Our tailored solutions meet the unique needs of researchers. With a commitment to quality, expertise, and customization, Lifeasible promises to be a trusted partner in advancing your plant research through scRNA-seq. If you are interested in our services or have some questions, please feel free to contact us or make an online inquiry.

Reference

1. Bawa G, Liu Z, Yu X, Qin A, Sun X. Single-Cell RNA Sequencing for Plant Research: Insights and Possible Benefits. Int J Mol Sci. 2022 Apr 19;23(9):4497.

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