DNA methylation, a chromatin modification present in plants and animals, is now well-studied and can be stably inherited both during cell division and, to some extent, during individual transmission. Different sequence contexts (CG, CHG, and CHH) and C in various types of motifs in plant genomes can be target sites for DNA methylation. Lifeasible can monitor DNA methylation at a high-resolution level by treating the DNA with sodium bisulfite followed by next-generation sequencing. Of course, we can do more than that.

Restriction endonuclease-based DNA methylation detection

We use restriction endonucleases as the basis for the restriction cleavage of methylated DNA or unmethylated DNA with one or more enzymes. We have combined this method with capillary sequencing, microarrays, and other technologies to detect genome-wide methylation levels in various organisms, limited to CpG sites recognized by endonucleases.

Bisulfite-based DNA methylation detection

We offer methylation detection and analysis by bisulfite modification, bisulfite-conjugated DNA direct sequencing, bisulfite-conjugated methylation-specific PCR, and combined bisulfite restriction analysis.

Immunoprecipitation of methylated DNA

We provide a one-stop methylation service, including ultrasound interruption of the genome, immunoprecipitation of methylated DNA, linear amplification of MeDIP and Input DNA fragments, fluorescent labeling, microarray hybridization, image acquisition, and data analysis.

SMRT sequencing for direct detection of DNA methylation

We offer SMRT to make single-molecule real-time sequencing possible, while providing an opportunity to explore methods for detecting base modifications. Our solution can be applied to regions that are difficult to sequence and can effectively improve the mining and processing of plant DNA methylation data for more biological information.

Single molecule nanopore dependent methylation sequencing

We offer single-molecule nanopore sequencing technology that enables you to detect endogenous CpG methylation and exogenous labeling of chromatin-accessible sites. To compensate for the lack of precision, we also offer a combined strategy of multi-platform sequencing, which brings you methylation information that cannot be found by a single sequencing tool.

RNA methylation

We provide machine learning-based RNA methylation modification site prediction and m6A methylation detection services to help you explore deeper information about RNA methylation modification in epigenetics. We have promoted RNA methylation modification analysis in several plant species, which can help you with studies on fruit ripening regulation, virus infection, increasing plant yield and biomass, abiotic resistance, and more.

RNA-directed DNA methylation

We can help you investigate the critical role of RdDM in plants for many processes, such as chromosome rearrangement, resistance to viral infection, regulation of gene expression, and development through genome-wide methylation sequencing and analysis of differentially methylated regions (DMRs).

DNA hydroxymethylation

We, combined with microarray technology and bioinformatic analysis, can obtain a genome-wide hydroxymethylation distribution map, which can help researchers to analyze the molecular mechanisms of species' morphological traits and stressful environmental adaptations from a new perspective.

Lifeasible always supports customization to try to meet your needs. If you are interested in any of the above options, feel free to contact us for more information.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

For research use only, not intended for any clinical use.

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