Plant proteomics studies all proteins expressed in the genome of a plant cell or tissue at a specific time or environment. As with other proteomics studies, sample preparation is the first and very important step in plant proteomics research. The main method of sample preparation is to fragment, lyse, inactivate, or reduce the sample such as cells or tissues, break the bonds between proteins as much as possible and extract all the proteins. Sample preparation is a key step in proteomics research and varies depending on the type of sample, the experimental objectives, and the analytical methods used. Lifeasible offers mass spectrometry-based plant proteomics analysis services, including plant proteomics sample preparation services.

What We Offer

• Plant Protein Sample Preparation

Plant cells contain thick cell walls, which makes it difficult to extract proteins from the samples. The number of biomolecules contained in plants varies greatly depending on the species and growth and development of the plant, and the number of biomolecules in plants is also closely related to the season. Currently, there are three methods commonly used by Lifeasible for the extraction of plant proteins: one is the common Tris-HCl extraction method, which dissolves as many soluble proteins in plants as possible by selecting an extraction buffer of appropriate pH. The other is the trichloroacetic acid/acetone precipitation method, in which trichloroacetic acid, as a protein denaturant, can effectively inhibit the activities of serine proteases and sulfhydryl protease activity and reduce protein loss. The third is the phenol/SDS protein extraction method, which can fully solubilize proteins in plant tissues in a shorter period by using phenol in the presence of the anionic surfactant SDS, and the obtained proteins are of higher purity.

Service Flow

Sample Requirement

• Whole plant material >2 plants
• Plant root, stem, leaf, and other parts of the tissue material >5 g

*For samples used for plant cultivation, please consult our staff to determine the sample conditions and dosage.

*The experimental samples will not be returned, please back them up by yourself. Before sampling, it is necessary to set up the control group and the experimental group, and each group is suggested to repeat more than 3 times.

*Quick-frozen preservation with liquid nitrogen can minimize the leakage time of plant samples at room temperature.

*There is no restriction on plant varieties. For varieties with special requirements or rare varieties, please contact our staff for more information.

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*If your organization requires the signing of a confidentiality agreement, please contact us by email.

For research use only, not intended for any clinical use.

Related Services

• Plant Protein MS Identification
• Plant Proteomics Analysis
• Quantitative Plant Proteome Analysis
• Plant Proteomic of Post-Translational Modifications
• Plant Exosome Proteomics