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In Vitro Organ Identification and Screening
The identification of disease resistance is an important foundation for disease resistance breeding, from antigen screening, progeny selection, to variety promotion. Lifeasible has established a variety of plant disease resistance identification and screening platforms, among which the in vitro organ identification screening method is to remove cotyledons, leaves, or fruits from plants for inoculation identification, which can identify those diseases with tissue, cellular, or molecular level resistance mechanisms. It can be used to identify diseases with resistance mechanisms at the tissue, cellular or molecular levels, such as potato blight, wheat powdery mildew, and tobacco black tibia. The in vitro identification method has the advantages of simple operation and reliable results, and can separately identify the resistance of the same material to different pathogens without affecting the normal growth and development of seedlings and flowering and fruit set.
What We Offer
Lifeasible can determine the disease resistance of a large amount of material to the disease in a relatively short period of time, and identify the resistance of different plant species to different diseases.
- Pathogen inoculation
Generally, the methods used are the smear method, a powder spray method, a spray method, injection method, etc. The methods used vary depending on the plant and the type of disease.
- Disease resistance identification
Disease resistance is usually measured by three indicators: infection type (IM, HR, R, MR, S, H), severity, and prevalence. The observations of the process include the presence and strength of allergic necrosis, whether spore mounds can be formed, and the size and number of spore mounds.
Sample Requirement
- Botanical material >10 g
*The experimental samples will not be returned, please back them up by yourself. Before sampling, it is necessary to set up the control group and the experimental group, and each group is suggested to repeat more than 3 times.
*Quick-frozen preservation with liquid nitrogen can minimize the leakage time of plant samples at room temperature.
*There is no restriction on plant varieties. For varieties with special requirements or rare varieties, please contact our staff for more information.
The services provided by Lifeasible cover all aspects of plant research, please contact us to find out how we can help you achieve the next research breakthrough.
Contact*If your organization requires the signing of a confidentiality agreement, please contact us by email.
For research use only, not intended for any clinical use.
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