Identification of Plant lncRNA

Our understanding of gene regulation is constantly evolving. It is now clear that most cellular transcripts are non-coding RNAs. The spectrum of non-coding RNAs is diverse and includes short (<200 nt) and long non-coding RNAs (lncRNAs) (>200 nt). LncRNAs regulate gene expression through diverse mechanisms. Many lncRNAs have been identified from plant species, including Arabidopsis thaliana, Medicago truncatula (barrel clover), Oryza sativa (rice), Petunia hybrida (petunia), Solanum lycopersicum (tomato), Triticum aestivum (wheat), Zea mays (corn), etc. Techniques commonly used to identify lncRNAs in plants are Microarrays and RNA-Seq.

Lifeasible, as a leading global company, is committed to helping our customers achieve effective and successful research. We provide analysis services for plant lncRNA identification, including sequencing, microarray, and bioinformatics analysis. In addition, we deliver reliable results and reports on time to our customers worldwide.

Plant lncRNA Sequencing

  • Although lncRNA transcripts make up the major part of the non-coding transcriptome, the discovery of lncRNAs is still at an early stage, and only a small fraction of lncRNAs have been studied so far. Developing high-throughput sequencing methods provides a means of quantifying the transcriptome by performing whole transcriptome sequencing or RNA sequencing.
  • We use next-generation high-throughput sequencing technology for lncRNA sequencing, combined with bioinformatics predictive tools for lncRNA analysis, to help our customers more quickly identify those lncRNAs with critical regulatory functions and analyze their relationships with specific biological processes.
Fig. 1 Pipelines of lncRNA identification.Fig. 1 Pipelines of lncRNA identification.

Plant lncRNA Microarray

  • Microarrays are widely used for gene expression analysis and preferred over RNA Seq for expression profiling of known transcripts because of their low cost (about 10 folds) and simplicity of data analysis. Based on probe design, microarrays are subdivided into non-tiling and tiling arrays.
  • We provide plant lncRNA microarray to systematically profile lncRNAs along with the entire set of protein-coding mRNAs, which is the best tool for profiling lncRNAs, overcoming many limitations of RNA-seq for lncRNAs often at low abundance.

Bioinformatics Analysis

  • Bioinformatics is defined as the application of tools of computation and analysis to the capture and interpretation of biological data. It is an interdisciplinary field which harnesses computer science, mathematics, physics, and biology. Bioinformatics is essential for the management of data in modern biology.
  • We provide bioinformatics services in plant lncRNA sequencing, including lncRNA prediction, lncRNA classification, differentially expressed lncRNA target gene enrichment, lncRNA and mRNA differential expression interaction analysis, etc. We also provide bioinformatics services in plant lncRNA microarrays, including differentially expressed lncRNAs with systematic annotation, identification of lncRNA enrichment in biological functions, and others.

Lifeasible is always devoted to providing high-quality and satisfactory service to our customers. If you are interested in our services or have questions, please contact us or make an online inquiry.

The services provided by Lifeasible cover all aspects of plant research, please contact us to find out how we can help you achieve the next research breakthrough.

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For research use only, not intended for any clinical use.

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