DNA methylation is a heritable epigenetic mark involving the covalent transfer of a methyl group to the C-5 position of the cytosine ring of DNA-by-DNA methyltransferases (DNMTs). In plants, cytosines are methylated in symmetrical (CG or CHG) or asymmetrical (CHH, where H is A, T, or C) contexts. Long non-coding RNA (lncRNAs) are classified as ncRNAs longer than 200 nt. They act at different levels of gene expression regulation, and one of these mechanisms is by recruitment of DNA methyltransferases or demethylases to regulate the target gene transcription.

Lifeasible provides analysis services for plant lncRNA in DNA methylation modifications to help our customers worldwide in plant science research. Our platform is equipped with cutting-edge facilities and professional experts to support research. Here, we provide various services according to customers’ demands.

DNA Methylation

• DNA cytosine methylation is an essential epigenetic modification that maintains genome integrity and regulates gene expression.
• We offer analysis services for plant lncRNA in DNA methylation modifications. DNA methylation in plants is mainly accomplished through the process of transcriptional silencing of genes due to RNA interference. And we help to analyze the role of DNA methyltransferases in DNA methylation modification.

LncRNAs in RdDM Silencing Pathway

• RNA-directed DNA methylation (RdDM) is the major small RNA-mediated epigenetic pathway in plants. RdDM requires specialized transcriptional machinery that comprises two plant-specific RNA polymerases, Pol IV and Pol V, and a growing number of accessory proteins.
• We provide analysis services of the role of plant lncRNA in the RdDM silencing pathway. This regulatory route is based on the performance of Pol IV-dependent RNAs (P4RNAs) transcribed by Pol IV. The lncRNA complex is driven to the chromatin target site together with a DNA methylation enzyme, and the DNA methyltransferase domains rearranged methyltransferase 2 (DRM2). This methyltransferase mediates de novo methylation of cytosines in all classes of sequence contexts at the target region to initiate gene silencing.

Fig. 1 The schematic process of DNA methylation.

• We help our clients analyze plant lncRNAs performing different developmental functions by participating in DNA methylation, including but not limited to regulating embryonic development, root organ development, reproduction, and gene silencing. In addition, we also analyze plant lncRNA in response to a wide range of abiotic stress by DNA methylation modification, such as the nutritional deficit, temperature, high salinity, and drought.

Lifeasible provides cost-effective, high-quality, and hassle-free services to our customers worldwide. ​We provide our clients with direct access to our experts and prompt responses to their questions. If you are interested in our services or have questions, please feel free to contact us or make an online inquiry.

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