RNA molecules in living organisms have a hierarchical structure, generally divided into the primary structure (also called sequence structure), secondary structure, and tertiary structure. Currently, it is difficult to obtain the tertiary structure of RNA, but there is partial evidence for the regulatory mechanism of lncRNAs through local single-stranded and local secondary structures. In recent years, many methods have been developed to assess the secondary structure of lncRNAs. This selection of methods can be divided into experimental and computational methods and include, but are not entirely limited to, enzymatic footprinting, chemical probing, nuclear magnetic resonance (NMR) spectroscopy, and comparative sequence analysis.

Lifeasible, as a leading global company, is committed to helping our customers achieve effective and successful research. We provide analysis services for plant lncRNA structure, including experimental, computational, and hybrid methods. In addition, we deliver reliable results and reports on time to our customers worldwide.

Structural Analysis of lncRNA by Experimental Methods

• Within the last decade, numerous different probing techniques evolved to explore the secondary structures of lncRNAs. These can generally be divided into enzymatic and chemical probing approaches, combined with high-throughput sequencing and consecutive bioinformatics analysis.
• We provide structural analysis services of lncRNA by experimental methods, including enzymatic footprint, chemical probing, nuclear magnetic resonance, small angle scattering, atomic force microscopy, cryo-electron microscopy, and others.

Fig. 1 Overview of integrative experimental approaches to analyze long non-coding RNA structure.

Structural Analysis of lncRNA by Computational Methods

• Since it was not feasible to experimentally determine the secondary structure of many long sequences, the in-silico prediction was the only realistic source for studying RNA structure for a long time.
• We provide structural analysis services of lncRNA by computational methods, including comparative sequence analysis, structure modeling, and others. Our algorithms for predicting secondary structure are sequence comparison methods based on pairing relationships between bases and thermodynamic minimum free energy methods based on complex structure formation by folding in RNA in vivo.

Structural Analysis of lncRNA by Hybrid Methods

• Experimental and computational methods alone may not fully capture the dynamic properties of lncRNA structure while combining complementary data from different methods improves the accuracy of structure prediction and overcomes the weaknesses of the methods applied alone.
• We provide structural analysis of lncRNAs through a hybrid approach, helping our customers select the appropriate structure determination and modeling method to match the characteristics of individual targets. Although, in general, not all experimental and computational methods are compatible with each other, we customize experimental plans to meet the specific research needs of our clients.

Lifeasible is always devoted to providing high-quality and satisfactory service to our customers. If you are interested in our services or have questions, please contact us or make an online inquiry.

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