Long non-coding RNAs (lncRNAs) are RNAs of more than 200 nucleotides in length that are not (or very rarely) translated into proteins with critical biological functions. For significantly differentially expressed lncRNAs obtained by microarray or RNA-seq sequencing, subsequent expression validation is required.

Lifeasible provides analysis services for plant lncRNA expression validation, including RT-qPCR, northern blot, rapid amplification of cDNA ends (RACE), and others, to help our customers worldwide in plant science research. Our platform is equipped with cutting-edge facilities and professional experts to support research. Here, we provide various services according to customers' demands.

RT-qPCR

• Real-time quantitative reverse transcription PCR or qRT-PCR is a technique used in molecular biology that enables reliable detection and measurement of products generated during each cycle of a PCR process. When using qRT-PCR to verify differential expression, it is essential to note that not all the PCR products reacting are the lncRNAs you want to study.
• We help customers analyze plant lncRNA expression validation by RT-qPCR, which includes the following workflows, RNA isolation, oligo (dT) primers annealing, first cDNA synthesis, denaturation, primer annealing and elongation, second cDNA synthesis, and detection.

Northern Blot

Fig. 1 Schematic representation of Northern Blot.

• The northern blot, or RNA bolt, is a laboratory method to detect specific RNA molecules among a mixture of RNA. This method can verify the lncRNA expression abundance and sequence information, but the precision is not as good as RT-qPCR.
• We provide analysis services for plant lncRNA expression validation by northern blot. We first use denaturing gel to separate RNA according to size. The RNA is then transferred to a nylon membrane while keeping the same distribution in the gel. After fixing the RNA to the membrane, a labeled probe complementary to the gene of interest is added to hybridize the immobilized RNA.

RACE

Fig. 2 Schematic diagram of RACE.

• RACE is a technique used in molecular biology to obtain the full-length sequence of an RNA transcript found within a cell. This methodology of amplification with single-sided specificity has been described by others as one-sided PCR or anchored PCR.
• We help customers to determine the lncRNA 5' and 3' end sequences by RACE technique and then obtain the full-length information of lncRNA. It includes the following workflows, isolation of RNA, design of 5'/3' RACE primers, first strand cDNA synthesis from total RNA, purification of first strand product, homopolymer tailing of cDNA, amplification of target cDNA, cloning 5'/3' RACE amplification products.

Lifeasible provides cost-effective, high-quality, and hassle-free services to our customers worldwide. We provide our clients with direct access to our experts and prompt responses to their questions. If you are interested in our services or have questions, please contact us or make an online inquiry.

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For research use only, not intended for any clinical use.

Related Services

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