Isobaric tags for relative and absolute quantitation (iTRAQ) technique and Tandem mass tags (TMT) technique use multiple (2-10) stable isotope tags and specifically label the amino groups of peptides for tandem mass spectrometry analysis, which can be used to compare the relative content of proteins in as many as 10 different samples at the same time. It can be used to study the differences in protein expression levels in tissue samples under different developmental stages. ITRAQ is an isotope labeling reagent. ITRAQ reagent consists of three parts: reporter group, mass balance group, and peptide reaction group, forming four or eight ectopic tags with equal relative molecular weight (145 or 305).

The same peptide from different samples had the same mass number after labeled with TMT/ iTRAQ reagent and showed the same mass peak in the first-order mass spectrometry (MS). When the mass peak is selected for fragmentation, in the secondary mass spectrometry (MS/MS), different reporter groups are released, and the signal intensity of their respective mass peaks represents the expression level of the peptide and its corresponding protein from different samples. TMT/ iTRAQ quantitative proteomics research has been widely used because of its high accuracy and adaptability to a wide variety of samples.

What We Offer

Based on traditional TMT/ iTRAQ analysis technology, Lifeasible provides TMT/ iTRAQ/ MultiNotch MS analysis technology and uses tertiary fragment report ions for quantitative analysis, which can further improve the accuracy of TMT/ iTRAQ quantitative proteomic analysis and quantitative accuracy. Lifeasible uses a high-resolution mass spectrometry system to launch quantitative proteome analysis service technology based on TMT/ iTRAQ/ MultiNotch MS. You only need to tell us the purpose of your experiment and send us your protein. We will be responsible for all the follow-up matters of the project, including protease digestion, peptide pricing, peptide separation, mass spectrometry analysis, mass spectrometry raw data analysis, bioinformatics analysis.

Service Flow

Technical Characteristics

• High protein throughput and coverage
• Accurate quantification with high confidence
• High separation capacity, wide analytical range, and reliable qualitative results
• The high degree of automation, fast analysis time, and good separation effect

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For research or industrial use.

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