Non-coding RNAs do not code for proteins, including miRNAs, lncRNAs, circRNAs, piRNAs, and so on. Non-coding RNAs function in many ways, interacting with proteins, DNA, and RNA, which participate in various cellular activities, mainly including activation and silencing of genes, splicing, modification and editing of RNAs, and translation of proteins, etc. Lifeasible can provide customers with direct markers to identify and provide some large-scale search technology solutions for non-coding RNA genes.

• We could identify non-coding RNAs by direct labeling simultaneously and by polyacrylamide gel separation, hierarchical separation of nuclear extracts, association with specific proteins, or overexpression of the dominant phenotype.
• We extracted all RNA from the material using RNA spiking, rather than based on DT priming and poly-A selection, and subsequently screened sequences of 50-500 nt in length and removed rRNA, tRNA, and clones of known non-coding RNA genes by high-throughput filter hybridization. Candidate genes for unknown non-coding RNAs were obtained after sequencing the remaining clones.
• We enriched non-coding RNA genes by immunoprecipitation reactions associated with specific kinds of non-coding RNA genes.
• There were also some non-coding RNA genes that we enriched by their 5' end characteristics that differed from the usual mRNA cap structure or by using subcellular localization.
• We use high-density oligonucleotide microarrays to detect all unknown transcripts to find non-coding RNA genes.

Among them, for lncRNA, we can perform lncRNA expression profiling of sample tissues by methods such as lncRNA microarray or RNA sequencing. We screen out lncRNAs with expression differences by bioinformatics methods, construct co-expression networks, and predict target genes of lncRNAs. We can get the lncRNA 5' full length by 5' RACE and the lncRNA 3' full length by 3' RACE, and finally get the complete lncRNA sequence. Candidate lncRNAs can also be validated by PCR or Northern Blot techniques to determine their expression differences.

Functional research

• Functional acquisition studies. We can construct non-coding RNA overexpression vectors.
• Loss-of-function study. We can silence non-coding RNAs by siRNA, shRNA, antisense nucleic acid, etc., and detect the effects on the expression of related genes and the cellular phenotype, etc., after interfering with non-coding RNAs.
• Expression regulation. We can detect DNA, RNA, and proteins bound to non-coding RNAs by RNA pull-down, RNA-RIP, ChIRP-seq, etc. Combine non-coding RNA expression with other fields to explain the mechanism of non-coding RNA regulation.
• Transcription factors. Study the regulatory mechanisms of non-coding RNAs and transcription factors.
• Chromatin remodeling. Non-coding RNA epistasis regulation.
• ceRNA mechanisms. We can help you study the regulatory mechanisms between the lncRNA-miRNA-mRNA triad.

Gene expression-based methods may miss certain non-coding RNA genes synthesized under certain conditions (e.g., certain cell types, certain periods, certain environmental conditions), so they are also known as small, temporary RNAs.Therefore, biological experiments for predicting non-coding RNA genes are often very complex and expensive, and the main problem is that the experimental timeframes are often quite long. Therefore, Lifeasible is constantly improving based on the protocols mentioned above, and we aim to provide our customers with proven protocols for non-coding RNA gene identification. Please feel free to contact us to submit your requirements so we can customize a solution for you promptly.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

For research use only, not intended for any clinical use.

Related Services

• Small RNA Regulation Analysis
• Small RNA Library Analysis
• Theoretical Predictions of Non-Coding RNA Genes
• MicroRNA Detection
• Small Interfering RNA
• RNA Modification Detection