The carbon fixed by plants through photosynthesis can be released into the soil in the form of root secretion, which provides rich nutrients for soil microorganisms. Soil microorganisms can colonize and reproduce in the root zone and root surface, which are rich in root secretions, by chemotaxis response. Soil microorganisms mainly include bacteria, actinomycetes, fungi, algae, protozoa, and viruses. Soil microorganisms are living organisms in the soil and are one of the most active soil fertility factors, and their zonal composition and quantitative changes usually reflect the level of soil biological activity. Changes in plant-microbe interactions mediated by root secretions are of great importance for soil fertility, health, and plant growth and development.

What We Offer

Microbial community diversity refers to the type and number of microbial populations, species richness and evenness, and species distribution in a community. Lifeasible commonly uses diversity indices (Simpson's diversity index, Shannon's diversity index, etc.), richness indices, and evenness indices to evaluate the structure and diversity of plant inter-root microbial communities. Lifeasible offers chemical or biological-based methods or modern molecular biology-based methods to provide inter-root microbiota analysis services.

• Chemical or biological-based methods

Traditional plate count methods, fluorescent staining methods, immunofluorescence techniques, community-level physiological profiling (e.g., biology microplate analysis), quinones profiling, and (phospholipid fatty acid analysis (PLFA)/fatty acid methyl ester analysis (FAME).

• Methods based on modern molecular biology techniques

Community-level total DNA analysis methods, such as community total DNA base composition percentage analysis methods. Molecular labeling methods based on molecular hybridization techniques, such as fluorescence in situ hybridization and integrated microscopic radioautography (MAR-FISH), isotope labeling techniques, DNA microarray. PCR-based methods, such as random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), restriction fragment length polymorphism (RFLP), terminal restriction fragment length polymorphism (T-RFLP), single-strand conformation polymorphism (SSCP), denaturing gradient gel electrophoresis (DGGE and TGGE), ribosomal intergenic region sequence analysis technique (RISA), etc. DNA or RNA sequence-based methods, such as metagenome sequencing, metatranscriptome sequencing, etc.

We Do Better

Lifeasible has been providing total solutions for plant inter-root biology for many years and has accumulated a wealth of technical experience. We are constantly improving our technical service platform by introducing more advanced technical services such as the soil metaproteomics method. Based on this method, we can provide plant inter-root biology and its molecular mechanism to help you discover more key soil proteins that mediate the plant-microbe inter-root "dialogue".

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For research use only, not intended for any clinical use.

Related Services

• Collection and Identification of Root Secretions
• Inter-rhizosphere Fungal Analysis
• Inter-rhizosphere Bacterial Analysis
• Plant Rhizosphere Actinomycete Analysis