MicroRNAs (miRNAs) and long non-coding RNAs (lncRNAs), two classes of endogenous non-coding RNAs, are critical regulators of gene expression in plants. As the two major types of ncRNAs, miRNAs, and lncRNAs play critical roles in plant growth, development, and biotic and abiotic stress responses. Recent studies have identified the interplay between miRNAs and lncRNAs as novel regulatory gene expression levels in plants. In addition to serving as targets or origins of miRNAs, some lncRNAs can regulate the biogenesis and function of miRNAs.

Lifeasible provides analysis services for plant lncRNA-miRNA interactions to help our customers worldwide in plant science research. Our platform is equipped with cutting-edge facilities and professional experts to support research. Here, we provide various services according to customers’ demands.

miRNA-Mediated Generation of phasiRNAs

• LncRNA transcripts can be targeted by miRNAs to generate phased small interfering RNAs (phasiRNAs).
• We provide analysis services for the miRNA-mediated generation of phasiRNAs. In phasiRNA biogenesis, RNAs, including lncRNAs are first typically cleaved by 22 nt miRNAs. Then the RNA-DEPENDENT RNA POLYMERASE6 (RDR6) recruited by AGO1-RISC or AGO7-RISC converts the 3’ fragment into double-stranded RNAs (dsRNAs), which are further processed by a Dicer protein to generate duplexes of phasiRNAs.

Fig. 1 Model diagram of lncRNA-miRNA interactions.

Regulating pri-miRNA Processing

• Natural antisense transcripts (NATs) belong to a class of coding or ncRNAs divided into two clades, cis-NATs, and trans-NATs, according to their derived region in the genome. Cis-NATs are transcribed from the opposite DNA strands at the same genomic locus, while trans-NATs originated from separate genomic loci.
• We help our customers analyze the interaction with plant lncRNA and miRNA. cis-NATs act as lncRNAs in the nucleus to impair the stability and processing of pri-miRNA without impacting their transcription. cis-NATs are widely present in plants and often affect the expression level of the associated sense genes.

Acting as Target Mimics of miRNAs

We offer analysis services for lncRNAs as miRNAs target mimics. Target mimicry is one of the most critical mechanisms of miRNA-lncRNA interplay, by which lncRNAs harboring endogenous target mimic (eTM) sites sequester miRNAs by sequence complementarity to inhibit their effects on target mRNA.

Inhibiting miRNA Expression

• Some nuclear-localized lncRNAs regulate gene transcription through mediating chromatin modification. They serve as a bridge between transcription factors and chromatin.
• We also help customers analyze lncRNA’s inhibitory effect on miRNA. LncRNAs act as a negative regulator by suppressing the expression of miRNA genes.

Lifeasible provides cost-effective, high-quality, and hassle-free services to our customers worldwide. ​We provide our clients with direct access to our experts and prompt responses to their questions. If you are interested in our services or have questions, please feel free to contact us or make an online inquiry.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

For research use only, not intended for any clinical use.

Related Services

• Plant lncRNA-mRNA Interactions Analysis
• Plant lncRNA-DNA Interactions Analysis
• Plant lncRNA-Protein Interactions Analysis