Long non-coding RNAs (lncRNAs) are a class of non-coding RNAs longer than 200 nt with high tissue specificities. They can be divided into intergenic, intronic, sense, and antisense lncRNA transcripts based on their relative position to coding genes. LncRNAs play vital roles in many important biological processes in plants. In terms of lncRNA conservation, almost all studies have come to the consistent conclusion that lncRNAs are very poorly conserved compared to protein-coding genes. The conservativeness of lncRNA can be studied mainly from 3 aspects, sequence, structure, and position.

Lifeasible provides analysis services for plant lncRNA conservation from these three aspects to help our customers worldwide in plant science research. Our platform is equipped with cutting-edge facilities and professional experts to support research. Here, we provide various services according to customers’ demands.

Sequence Conservation Analysis

• In terms of sequence conservativeness, the low conservativeness of lncRNA sequences is only in comparison to protein-coding gene sequences. Compared to intronic and intergenic sequences, lncRNA sequences are still highly conserved.
• We provide analysis services for plant lncRNA sequence conservation. With the recent development of transcriptome sequencing technology, the analysis of plant lncRNA sequence conservation has entered the era of hyper-scale. We use all the IncRNAs derived from the customers as the query dataset and searched against other IncRNAs and genome sequences with BLAT software. The best alignments are selected and used in the statistical analysis.

Structural Conservation Analysis

• On structural conservation, when homology is defined using sequence conservation alone and homologous gene pairs between species cannot be fully identified, it is possible to find these missing homologs through structural conservation. However, there is no evidence to support this claim so far.
• We help our customers analyze the structural conservation of plant lncRNA. Although genome-wide analysis provides limited support for finding selective pressure to maintain secondary structure in lncRNAs, it is not a substitute for structure-based homology search for lncRNA homology detection.

Position Conservation Analysis

Fig. 1 Venn diagram of lncRNAs showed positional conservation.

• In terms of positional conservation, a significant number of lncRNAs are positively conserved when comparing distantly related species, i.e., have the same relative orientation as homologous protein-coding genes and/or other conserved regions, and most of them are not of detectable sequence homology.
• We also offer analysis services for plant lncRNA position conservation. These positionally conserved gene pairs may correspond to lncRNAs whose functional sequences are too short or for which sequence homology cannot be detected or to lncRNAs whose transcriptional action alone is under selective pressure. Therefore, positional conservatism is a powerful complement to the study of lncRNA conservatism.

Lifeasible provides cost-effective, high-quality, and hassle-free services to our customers worldwide. ​We provide our clients with direct access to our experts and prompt responses to their questions. If you are interested in our services or have questions, please feel free to contact us or make an online inquiry.

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Related Services

• Plant lncRNA Specificity Analysis