With the advances in high-throughput sequencing and other experimental techniques, more ncRNAs reveal their functional roles. They have been shown to play a key role in regulating gene expressions at epigenetic, transcriptional, posttranscriptional, and posttranslational levels. lncRNAs can regulate the expression of close or distant genes. The reported mechanisms involved include the recruitment, interaction with, and modulation of chromatin-modifying complexes or DNA-binding proteins but also modifications of the epigenetic properties of chromatin and its structure.

Lifeasible provides analysis services for plant lncRNA-DNA interactions to help our customers worldwide in plant science research. Our platform is equipped with cutting-edge facilities and professional experts to support research. Here, we provide various services according to customers’ demands.

Plant lncRNA-DNA Binding for Modulating Chromatin Loop Dynamics

• A chromatin loop occurs when stretches of genomic sequence that lie on the same chromosome (configured in cis) are in closer physical proximity to each other than to intervening sequences. So, dynamic chromatin topology is closely associated with gene expression patterns.
• We provide analysis services of plant lncRNA-DNA binding for modulating chromatin loop dynamics. lncRNAs can regulate cis or trans-responsive genes. In addition, lncRNAs recognize their distant target genes by base complementation through the formation of DNA-RNA duplexes.

Fig. 1 Schematic representation of lncRNA-DNA interactions. (Chen Q, et al., 2021)

Plant lncRNA-DNA Binding for Modulating Gene Transcriptions

• Although thousands of lncRNAs have been systematically identified or predicted in silico, very few have been characterized functionally. The lncRNAs can interact with DNA to regulate gene transcriptions.
• We help customers to analyze this lncRNA-DNA binding interaction. Plant lncRNAs bind to the proximal promoter of the binding gene and repress its expression.

Transcription of Plant lncRNA for Modulating DNA Transcriptions

• In plants, a novel transcriptional regulation mechanism of coding genes depends on the transcription of the adjacent lncRNA.
• We help our customers analyze the interaction with plant lncRNA and mRNA. When sense DNA is initiated, lncRNA is induced in the antisense region of DNA. Then, RNA Pol II read-through transcription of lncRNA leads to the expression of antisense DNA and an increase of RNA Pol II occupancy on strands. The collision of RNA Pol II stalls DNA sense transcription.

Our Technical Support

We provide advanced, high-quality, well-established technical support to help our customer’s study plant lncRNA-DNA interactions, including but not limited to ssDRIP-Seq (single-stranded DNA-RNA hybrid immunoprecipitation), DRIP-RNA-Seq, R-CHIP (high-resolution R-loops genome-wide mapping), RIDP (RNA isolation by DNA purification), and others.

Lifeasible provides cost-effective, high-quality, and hassle-free services to our customers worldwide. ​We provide our clients with direct access to our experts and prompt responses to their questions. If you are interested in our services or have questions, please feel free to contact us or make an online inquiry.

Reference

1. Chen Q, et al. (2021). “From "Dark Matter" to "Star": Insight Into the Regulation Mechanisms of Plant Functional Long Non-Coding RNAs.” Front Plant

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Related Services

• Plant lncRNA-miRNA Interactions Analysis
• Plant lncRNA-mRNA Interactions Analysis
• Plant lncRNA-Protein Interactions Analysis