Targeted next-generation sequencing (tNGS) is a next-generation sequencing technology based on the combination of ultra-multiplex PCR amplification and high-throughput sequencing. It does not rely on traditional microbial culture, but directly enriches dozens of known pathogenic microorganisms in plant samples, and then performs high-throughput sequencing, which can be compared with the database to determine the species of pathogenic microorganisms contained in the samples. The sequencing is then compared with a database to determine the type of pathogenic microorganisms contained in the sample based on the sequence information obtained from the comparison. This technology allows for the rapid and objective detection of a large number of pathogenic microorganisms in a sample and is particularly advantageous for the detection of low concentrations of pathogenic microorganisms.

Lifeasible has extensive experience in the field of botanicals, and with our advanced technology platform and experienced technical team, we provide high-quality pathogen-targeted sequencing (tNGS) to help researchers advance the detection of pathogenic microorganisms in plant samples. With our unique technology platform and quality standard service process, we can fully meet all your needs for plant research.

Advantages of targeted next-generation sequencing (tNGS)

• Cheaper. Since tNGS can directly sequence the targeted gene, the amount of sequencing data required is smaller, making sequencing faster and cheaper in comparison.
• More sensitive. Since tNGS uses specific primers or probes to enrich for nucleic acids of target microorganisms before sequencing, it can detect very low concentrations of pathogenic microorganisms.
• High sensitivity and specificity. tNGS can design customized probes for known or suspected pathogens, dramatically improving the accuracy of the assay.

What do we offer for tNGS?

• Nucleic acid extraction. Total DNA or RNA is extracted from plant samples using appropriate extraction methods, and the concentration and purity of the extracted product are checked to ensure that it meets the sequencing requirements.
• Targeted sequence amplification. We design primers or probes specific for known or suspected pathogens and amplify the target sequences by PCR or other amplification techniques.
• Library construction. We then construct high-throughput sequencing libraries by end-repairing and ligating the amplified products. Quantification and quality control of the library to ensure that it meets the requirements of the sequencing instrument.
• High-throughput sequencing. The constructed sequencing library will be uploaded to the machine for high-throughput sequencing. Through the high-throughput sequencing platform, obtain the sequence information of nucleic acids after target capture.
• Data analysis. We pre-processed the sequencing data for quality contro, splicing, and comparison. We also used bioinformatics software or customized processes to classify and identify the sequencing reads and determine the pathogen species present in the samples.
• Analysis of results. Based on the analysis results, we can help researchers determine the pathogen information in plant samples and provide a basis and guidance for subsequent disease monitoring, diagnosis, and control.

Significance of tNGS

• Pathogen detection. The tNGS technology can rapidly and accurately detect the presence of bacteria, viruses, fungi, and other pathogens in the plant body, which can provide the basis for the subsequent prevention and control measures.
• Disease monitoring. By applying tNGS technology to large-scale field surveys, we can effectively monitor the dynamic changes of plant diseases and provide data support for scientific research and disease early warning.
• Pathogenesis research. By sequencing and analyzing the genomic information of pathogens, the pathogenesis of pathogens can be investigated in depth, laying the foundation for the development of targeted prevention and control measures.
• Identification of disease resistance. Utilizing tNGS technology to detect the status of plant disease resistance genes, providing molecular marker information for disease resistance breeding.

Why choose us?

• One-stop service. We provide customers with project development, and technical support to the report analysis of the whole process of services, to ensure that customers save more time for research.
• Extensive technical cooperation. We have established close technical cooperation with many famous universities and research institutes and can provide advanced and reliable technical support and consulting services for our clients.
• Rapid response customer service. We have established an efficient project management mechanism, which enables us to respond quickly to customers' needs, provide timely feedback on the progress of experiments and analysis of results, and ensure that customers can obtain satisfactory technical services promptly.
• Advanced technology platform. We are always at the forefront of industry development to ensure that we provide the most advanced technical services to meet our client's research needs.

Lifeasible has established a well-established system in the basic field of botany and also provides technical support for agricultural development. We actively explore the latest research methods and have several partners and many customers around the world. We aim to work with plant scientists to advance plant research. If you are interested in us, please feel free to contact us.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

For research use only, not intended for any clinical use.

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