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Immunoprecipitation of Methylated DNA
DNA methylation is an essential component of epigenetics, which plays a vital role in maintaining normal cell function, genetic imprinting, cell development, and other processes. It is one of the new research hot spots. With the increasing research on methylation, various methylation detection methods have emerged. The methylated DNA immunoprecipitation (MeDIP) method enriches genomic unmethylated or methylated fragments by immunoprecipitation with proteins containing methyl-binding structural domains or with 5mC-specific antibodies. After finally obtaining purified methylated DNA fragments, Lifeasible can be combined with existing microarray technology to detect gene methylation or with fluorescent quantitative PCR technology to quantify DNA methylation.
MeDIP coupled with real-time fluorescent quantitative PCR (MeDIP-qPCR)
qPCR is a method of adding fluorescent reagents to the PCR reaction system, detecting the PCR process in real-time using the accumulation of fluorescent signals, and finally quantitatively analyzing the unknown templates using a standard curve. qPCR can accurately quantify the concentration of the starting template in a sensitive, rapid, specific, and highly reproducible manner. Combining MeDIP and qPCR, we first enrich methylated DNA fragments with MeDIP and then detect them by qPCR. After data analysis and processing, we can obtain precise values of methylation levels of specific sites in the detected biological samples. Our method has the advantages of no need for labeled probes, high specificity, high sensitivity, high accuracy, and good reproducibility.
MeDIP-chip technology
MeDIP-chip is currently an accurate and reliable experimental technique for high-throughput analysis of genomic DNA methylation changes. Using this technology, we can detect methylation sites on a genome-wide scale, helping you to study the regulation of genes by promoter methylation, compare methylation profiles of different tissues, cells, etc., as well as search for molecular markers.
We keep abreast of the forefront of epigenetic research and have developed a complete, reliable, and advanced experimental system that can help you with pre-immunoprecipitation experiments, microarray experiments, and post-analysis of data.
Lifeasible provides a one-stop methylation service, you only need to provide well-preserved tissue or cell specimens, and our technical service personnel can complete all the experimental operations for you, including ultrasound interruption of the genome, immunoprecipitation of methylated DNA, linear amplification of MeDIP and Input DNA fragments, fluorescent labeling, microarray hybridization, image acquisition and data analysis, and provision of complete lab reports. Please feel free to contact us for your customized protocol.
The services provided by Lifeasible cover all aspects of plant research, please contact us to find out how we can help you achieve the next research breakthrough.
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