Non-coding RNAs (ncRNAs) constitute a group of genetic information-containing molecules generated from in vivo genome transcription but do not encode proteins. Long ncRNAs (lncRNAs) are a class of transcripts with low sequence conservation in different species, a length of over 200 nucleotides, and no protein-coding ability. Once considered as 'dark matter' or 'transcriptional noise' in the genome, lncRNA has become one of the main topics of epigenetic research.

Lifeasible, as a leading global company, is committed to helping our customers achieve effective and successful research. We provide analysis services for plant lncRNA identification, structure, functions, and verification. In addition, we deliver reliable results and reports on time to our customers worldwide.

Identification of Plant lncRNA

• The development of high throughput technologies has aided the in-silico identification and characterization of plant lncRNAs. However, the difficulty in separating the coding from the non-coding RNAs in the thousands of assembled transcripts makes identifying lncRNAs very challenging.
• As the technology continues to mature, many lncRNAs have been identified from plant species, including barrel clover, rice, tomato, wheat, corn, etc. We have developed several methods to identify lncRNA in plants, such as RNA-sequencing (RNA-Seq), deep RNA-Seq, strand-specific RNA-Seq, microarray, and others. Also, we provide complete and detailed reporting of experiments and data.

Analysis of Plant lncRNA Structure

• LncRNAs are often defined as non-coding RNAs greater than 200 nucleotides in length, transcribed by different polymerases, with mRNA-like poly-A tails and promoter structures after the cap structure and polyadenylation are removed.
• Currently, it is difficult to obtain the tertiary structure of RNA, but there is partial evidence for the regulatory mechanism of lncRNAs through local single-stranded and local secondary structures.
• It is feasible to study the functional mechanisms of lncRNAs by combining the secondary structure features. Therefore, we provide structural analysis services of plant lncRNA for our clients, often using X-ray imaging, NMR techniques, chemical reagents, calculation predictions, and others.

Analysis of Plant lncRNA Functions

• Molecular detection and phenotypic comparison analysis of transgenic plants with stable expression through genetic transformation are the gold standards for gene function verification.
• We offer plant lncRNA functional validation services using a strategy based on acquisition/loss of function, i.e., lncRNA overexpression studies based on genetic transformation platforms or loss of function studies based on clustered regularly interspaced short palindromic repeat (CRISPR) related protein 9 (Cas9) systems, RNAi, and others.

Analysis of Plant lncRNA Verification

We also provide comprehensive plant lncRNA validation analysis services, including expression, localization, non-coding ability, and reciprocity validation.

Lifeasible is always devoted to providing high-quality and satisfactory service to our customers. If you are interested in our services or have some questions, please feel free to contact us or make an online inquiry.

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Related Services

• Analysis of Plant lncRNA Biology
• Analysis of Plant lncRNA Interactions
• Analysis of Plant lncRNA Regulation
• Analysis of Plant lncRNA Biofunctions
• Analysis of Stress Response of Plant lncRNA