Species identification (strain identification) by DNA sequencing and various molecular biology methods is a more advanced identification method than traditional biochemical identification and is faster and more accurate than traditional biochemical identification for all strains. Lifeasible offers a wide range of microbial strain identification methods.

What We Offer

• Routine identification

Lifeasible provides routine characterization including morphological and physiological and biochemical characteristics. Morphological characteristics include microscopic morphology and culture characteristics, while physical and chemical characteristics include nutrient type, carbon, and nitrogen source utilization capacity, various metabolic reactions, enzymatic reactions, etc.

• Automatic carbon source analysis and identification

Lifeasible can test the fingerprint of microorganisms based on their utilization of different carbon sources, establish a database corresponding to the microorganism species, and compare the microorganisms to be tested with the database through software to obtain identification results.

• Molecular biology identification

The molecular biology method is the most common method used in microbial taxonomy research to elucidate the taxonomic relationships among microbial populations from the perspective of genetic evolution. The laboratory is equipped with advanced instruments and equipment such as PCR, high-speed freezing centrifuge, electrophoresis, HPLC, gel imaging system, UV temperature control system, and sequence analysis software such as DNAMAN, BIOEDIT, CLUSTALX, and TREEVIEW. Currently, Lifeasible can analyze bacterial 16 S rDNA/16 S-23 S rDNA interval sequences, yeast 18 S rDNA/26 S rDNA (D1/D2) sequences, and filamentous fungi 18 S rDNA/ ITS1-5. 8S-ITS2 sequences by nucleic acid sequence analysis to provide scientific identification results.

• Bacterial numerical identification

The Lifeasible identification system covers 15 identification series with approximately 1000 biochemical reactions and currently identifies over 600 species of bacteria. During the identification process, the appropriate physiological and biochemical identification series can be selected according to the taxon to which the bacteria belong, and the results can be obtained by comparing the bacteria to be tested with the database through the software.

• RAPD, SSCP technology

Lifeasible uses Randomly Amplified Polymorphism DNA (RAPD) and Single-Strand Conformation Polymorphism (SSCP) techniques for the identification of microbial strains.

• TLC thin layer chromatography

Lifeasible applied TLC thin layer chromatography for microbial strain identification, and performed an analysis of chemical fractions (amino acids, sugars) of bacterial and actinomycete cell walls as an important identification technique to characterize the genus, and played a good supporting role.

• Whole-cell fatty acid analysis and identification

Lifeasible uses a fully automated bacterial identification system to identify bacteria and yeasts by analyzing the fatty acid profiles of different strains and comparing them with a standard database. This technique is an effective tool for identifying bacteria or yeast at the species level.

• (G+C) mol% and DNA/DNA hybridization

Lifeasible uses a nucleic acid-protein analyzer to determine the Tm value to obtain the (G+C) mol% of microbial strains and analyze DNA/DNA homology with the model strain, an important component of multi-phase identification.

• Real-time fluorescence quantitative PCR

The real-time fluorescence quantitative PCR instrument is an integrated system for specific target gene detection and quantification, which combines PCR thermal cycling, fluorescence detection, and various application analysis software, allowing dynamic observation of the gradual increase of PCR amplification products in each reaction tube for each cycle of PCR. Compared with conventional PCR, it has more specificity, effectively solves the problem of PCR contamination, and has a higher degree of automation.

• Microbial colony analysis

This technique provides information on the dominant microbial species in a community and analyzes multiple samples simultaneously, is reproducible and simple, is suitable for investigating spatial and temporal variation in populations, and can be used to identify community composition through sequence analysis of bands or hybridization with specific probes.

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For research use only, not intended for any clinical use.

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