LncRNAs are an abundant class of RNAs >200 nt in length previously assumed to be transcriptional noise. While tens of thousands of lncRNA transcripts have been cataloged, the biological function of most lncRNAs remains a mystery. Determining the individual functionality of members of this recently discovered class of RNAs is essential for a better understanding of developmental biology and evolution.

Lifeasible provides knockdown analyses of plant lncRNA, including antisense oligonucleotides, site inversion, and promoter deficiency. Our platform is equipped with cutting-edge facilities and professional experts to support research. Here, we provide various services according to customers' demands.

Antisense Oligonucleotides

• Antisense oligonucleotides (ASOs) are a type of chemically synthesized short single-stranded oligonucleotides that have a 15- to 25-nucleotide DNA sequence. They can bind to complementary RNA and recruit RNase H, provoking RNA degradation and altering the expression of downstream proteins. Moreover, they can also inhibit transcription without the assistance of RNase H cleavage, such as by the spatial blocking of ribosomes.

Fig. 1 Schematic diagram of antisense oligonucleotide knockdown of lncRNA.

• We help our customers to perform functional analysis with antisense oligonucleotides, including cloning the full-length transcript of lncRNA and defining its testicular localization pattern. Then, we provide the delivery system to the plant according to our customer's research.

Locus Deletion

• If removing a complete lncRNA locus does not result in subtle effects on gene expression; this locus can be marked non-functional, at least in the analyzed biological system. This crude approach eliminates any transcript coming from the locus, eliminating the possibility that degradation of any residual transcript does cause any effect by, for example, genetic compensation.
• We provide locus deletion services to knock down lncRNA. The fragment containing a full-length lncRNA sequence is amplified by PCR and inserted into the vector. PCR-based deletion strategy is used to generate the deletion mutant. Our services also include designing primer and the delivery system into the plant according to the specific research.

Promoter Deletion/Inversion

• All gene expression requires a promoter for transcription, and a missing promoter is a loss of transcriptional activity and cannot be expressed. So, promoter deletion/inversion analysis is useful for identifying important regulatory regions involved in the transcriptional control of gene expression.
• We help our customers perform promoter deletion and/or inversion to analyze the function of lncRNA. In addition, we also perform knockout validation and select stably expressed plants for downstream functional experiments.

Lifeasible provides cost-effective, high-quality, and hassle-free services to our customers worldwide. We provide our clients with direct access to our experts and prompt responses to their questions. If you are interested in our services or have questions, please contact us or make an online inquiry.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

For research use only, not intended for any clinical use.

Related Services

• Plant lncRNA Gain-of-Function Analysis
• Plant lncRNA Loss-Of-Function Analysis
• Functional Analysis of Plant lncRNA with CRISPR-Cas Technology