Once considered as "dark matter" or "transcriptional noise" in the genome, lncRNA has become one of the main topics of epigenetic research. Whole-genome and transcriptome sequencing results have shown that more than 75% of the human genome and more than 50% of the Arabidopsis thaliana genome could be transcribed into RNA. The abundance of lncRNA expression in plants is low, generally only 1/30 to 1/60 of the average mRNA expression. Transient expression in protoplasts and RNA FISH can verify the subcellular localization of lncRNAs, and the subcellular localization correlates with the regulatory mechanisms and functions of lncRNAs.

Lifeasible provides analysis services for plant lncRNA subcellular localization validation, including fluorescence in situ hybridization (FISH), prediction model, and others, to help our customers worldwide in plant science research. Our platform is equipped with cutting-edge facilities and professional experts to support research. Here, we provide various services according to customers' demands.

Transient Expression and FISH

• Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that allows the localization of a specific RNA sequence or an entire chromosome in a cell, which provides researchers with a way to visualize and map the genetic material in an individual's cells, including specific genes or portions of genes.
• The plant protoplast contains all the parts of a cell without a cell wall and is a complete "naked cell" with physiological, biochemical, and metabolic activities. Plant protoplasts provide a multifunctional cell-based experimental platform. The most significant advantage of transient expression in protoplasts is rapid detection and high throughput.

Fig. 1 Basic steps of fluorescent in situ hybridization technique.

• We help customers analyze plant lncRNA localization validation by transient expression in protoplasts based on a tobacco transient transformation system and FISH. The workflow of FISH generally includes the following workflows, denaturation of probes, denaturation of specimens, hybridization, elution, amplification of hybridization signals, blocking of slices, and observation of results by fluorescence microscopy.
• In addition, we also offer different types of hybridization probes according to the research's purposes, such as chromosome-specific repeat sequence probes, whole chromosome or chromosome region-specific probes, and position-specific probes. Fluorescein labeling of the probes can also be done by direct or indirect labeling.

Prediction Model

• LncRNA subcellular localization is highly informative regarding its biological function, although it is challenging to discover because few prediction methods currently exist.
• We help our clients develop the prediction model for lncRNA subcellular localization directly from lncRNA transcript sequences in plants. In addition, we help evaluate the model's accuracy, specificity, and sensitivity.

Lifeasible provides cost-effective, high-quality, and hassle-free services to our customers worldwide. We provide our clients with direct access to our experts and prompt responses to their questions. If you are interested in our services or have questions, please contact us or make an online inquiry.

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Related Services

• Plant lncRNA Expression Validation Analysis
• Plant lncRNA Non-Coding Ability Validation Analysis
• Plant lncRNA Interaction Validation Analysis