Genetic mapping involves the development of many molecular markers based on whole genome sequencing or simplified genome sequencing. These markers are used to determine the relative positions of genes on the same chromosome and map the chain according to the law of recombination exchange. Genetic mapping constructed by high-throughput sequencing has higher mapping resolution and more accurate QTL localization. Lifeasible can accelerate the mining of critical trait genes in coffee by genetic mapping construction, which is an effective method for accelerating coffee breeding.

Our coffee genetic mapping construction can be applied to the following studies

• QTL localization and functional mining
• Assisted genome assembly
• Comparative genomics analysis
• Molecular marker-assisted breeding

We provide effective solutions for coffee genetic mapping

As part of our coffee genetic map construction service, we use high-throughput sequencing technology to build a high-density genetic map to improve localization accuracy, accelerate the process of functional gene discovery and verification, and provide coffee genome evolution information.

We provide the following methods for coffee genetic profiling

• High-density genetic mapping. Based on the exchange and recombination of chromosomes in chain genetic inheritance, we calculate the exchange value by multiple consecutive two-point or three-point tests, determine the distance of relative positions of genes located on the same chromosome, and plot the chain genetic map.
• Polyploidy and genetic mapping. Polyploidy is one of the evolutionary drivers of plants, and the construction of genetic maps for polyploid species, which have been more challenging to decipher based on their advantages in growth vigor, biomass accumulation, and resistance adaptations, is much more complex compared to diploids.

Features of our technology

• Reduces the complexity of the genome to a large extent and facilitates genome assembly.
• Species-independent, used for both germline and non-germline species.
• High-throughput sequencing can effectively develop SSR and InDel markers between two parents while detecting SV and CNV variation information.

Our service workflow

Advantages of our services

• High efficiency and quality. Based on our whole genome resequencing or simplified genome sequencing assays, many molecular markers can be developed rapidly.
• High-quality sequencing data, super-performance computing platform. We have an advanced high-throughput sequencing platform which can read high-quality sequencing data quickly and efficiently.
• Rich project service experience. So far, we have completed the genetic mapping of several coffee varieties.
• Scientific program design. Each step of our experimental operation is scientifically and meticulously designed to ensure high-quality research results.

Lifeasible adheres to the concept of "scientific protocol design, strict quality control management, professional analysis team, rich project experience, and quality project service" to ensure that each step is completed with excellence and helps scientific research. If you are interested in us, please feel free to contact us.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

For research use only, not intended for any clinical use.

Related Services

• Decaffeinated Coffee Breeding
• Coffee Varieties Improvement
• Identification of Coffee Germplasm Resources
• Genetic Diversity Analysis of Coffee
• Coffee Genetic Stability Analysis