Epigenetic of Plant Centromere

Plant mitoplasts are the most evolutionary and structurally complex regions of the genome. Most plant mitotic grains are structurally complex, consisting mainly of highly repetitive satellite DNA with interspersed retrotransposon sequences (CRs). They are important in species formation and differentiation and are structurally complex and epigenetically regulated. Lifeasible provides services such as mitotic DNA sequence cloning and isolation to support your in-depth study of how mitotic DNA sequences are involved in mitotic function, evolution, and epigenetic regulation.

Epigenetic of plant centromere.

Centromere DNA sequence cloning and isolation

Separation method		Centromere DNA sequences
CsCl density gradient centrifugation	This method allows us to isolate centromere repeat sequences for use in centromere repeat screening in species with no basis for inbreeding studies.	Satellite DNA
Genomic DNA digested with restriction endonucleases	Restriction endonucleases digest genomic DNA with a single cleavage site within the repeat unit, and the centromere tandem repeats appear as bright, clearly delineated linear fragments on electrophoresis. We perform recovery and identification to obtain centromere highly repetitive DNA sequences.	Tandem repeat
Microdissection and microcloning	We cut and separate chromosomes or fragments under a microscopic operating system. We can also combine chromosome microdissection with PCR, micro cloning, FISH, library screening, and other techniques.	Centromere region of B Chromosome Satellite chromosomes Telocentric chromosome Chromosome 1
The screen of BAC library	For species with completed genome sequencing, we can screen for BACs in the near-centromere region by end-sequencing. For species with known centromere DNA sequences in close relatives, we can screen using methods such as colony hybridization and Southern. For species with existing genetic maps, we can perform pooled PCR screening using near-centromere markers in the maps. For species with no centromere research base, we can use genomic colony hybridization to screen for BACs containing highly repetitive sequences and then further screen for centromere-associated sequences.	Centromere BAC
Centromere binding protein	We use CenH3-specific antibodies to specifically precipitate and isolate DNA sequences that bind to CenH3 and screen for highly repetitive sequences by bioinformatics analysis.	Spindle fiber attachment (SFA) Tandem repeat Retrotransposon

Centromere FISH marker screening

We can help you screen clones located in the centromere region in specific libraries as centromere FISH probes for constructing species-specific molecular cytogenetic profiles.

In addition to the above, Lifeasible can provide you with a complete assembly of centromere, telomeric repeat cluster tagging, centromere array tagging, and cytogenetic validation of chromosome-specific satellites using FISH for cytogenetic validation of centromere according to your needs for comprehensive analysis of the genetic, structural and functional, and epigenetic characteristics of centromere. Please feel free to contact our staff for more information.

The services provided by Lifeasible cover all aspects of plant research, please contact us to find out how we can help you achieve the next research breakthrough.

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