The phospholipid is a constituent of cells and is widely distributed in plants, with a wide range of important physiological effects. Because of its unique physical and chemical properties, it can be used as a nutritional additive, emulsifier, and shortening agent in pharmaceutical, food, and daily chemical industries, as well as in the production of detergents and coatings. Phospholipids include phosphatidylcholine (lecithin, PC), phosphatidylethanolamine (ceruloplasmin, PE), phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidylglycerol (PA), and their derivatives.

What We Offer

Lifeasible provides extraction, determination, and analysis of plant phospholipids. The extraction of plant phospholipids is mainly done by organic solvent extraction and supercritical CO₂ extraction. By extracting impurities such as oil and grease, pure powdered phospholipids with acetone insoluble matter content of 95% or more are obtained. The general extraction process includes oil gel, pretreatment, extraction, and purification. In the experimental process, high-performance liquid chromatography (HPIC), molybdenum blue colorimetry, spectrophotometry, nuclear magnetic resonance (NMR), infrared spectroscopy, thin-layer chromatography (TLC), HPLC-ELSD, and chromatography-mass spectrometry (GC-MS) were used to determine the components and contents of phospholipids, including the determination of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylinositol (PI).

Methods	Characteristics
Molybdenum blue colorimetric method	The method is simple and inexpensive, and the results are accurate but can be easily influenced by other organic or inorganic phosphorus-containing substances that are not phospholipids.
Spectrophotometric method	It has high precision, does not require expensive instruments, and is relatively simple to operate.
NMR	It can analyze the composition and content of various phospholipids, with fast and sensitive detection, low interference factors, high accuracy, and penetration into the measured material without destroying it, but this method requires high sample purity and high sample demand.
Infrared spectrometry	Compared with the molybdenum blue colorimetric method, its results are correct, stable, rapid, convenient, and sensitive.
TLC	This method is fast and sensitive, has a wide analytical range, and requires a small sample size, but is subject to many external conditions, and standards are not easily available for quantitative determination.
HPLC-ELSD	The mobile phase is evaporated before entering the detector, so it does not affect the final results.
GC-MS	GC-MS is the most accurate means of determining the molecular weight of phospholipids, with high separation power, sensitivity, and selectivity, plus the system provides a control spectrum.

We Do Better

Lifeasible provides simple and efficient separation and analysis of phospholipid fractions. With our experience and a wide range of extraction and analysis methods, we provide high-quality monitoring and analysis services for plant phospholipids to our customers worldwide.

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For research use only, not intended for any clinical use.

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