Long non-coding RNAs (lncRNAs) are abundant in eukaryotes and have various biological functions, and their action mechanisms are complex and diverse. LncRNAs are a group of poorly conserved RNA molecules with a sequence length of more than 200 nucleotides and no protein encoding capability; they interact with large molecules, such as DNA, RNA, and proteins, and regulate protein modification, chromatin remodeling, protein functional activity, and RNA metabolism in vivo through cis- or trans-activation at the transcriptional, post-transcriptional, and epigenetic levels.

Lifeasible provides analysis services for plant lncRNA interaction validation, including RNA immunoprecipitation (RIP), RNA-pull down, electrophoretic mobility shift assay (EMSA), and others, to help our customers worldwide in plant science research. Our platform is equipped with cutting-edge facilities and professional experts to support research. Here, we provide various services according to customers' demands.

RIP

• RNA immunoprecipitation (RIP) is a powerful method to study the physical association between individual proteins and RNA molecules in vivo. The basic principle of RIP is very similar to chromatin immunoprecipitation (ChIP), which uses target proteins to bind RNA to form a precipitated complex, and purifies RNA from the precipitated RNA-protein complex for quantitative or sequencing detection.

Fig. 1 Schematic diagram of RIP technology.

• We provide analysis services for plant lncRNA interaction validation by RIP, an essential tool for studying RNA-protein interactions and proving whether proteins interact with lncRNAs. Its workflow includes harvesting cells, isolation nuclei and lyse nuclear pellets, shear chromatin, immunoprecipitated the RNA binding protein (RBP) of interest together with the bound RNA, washing off unbound material, purifying RNA that is bound to immunoprecipitated RBP, reversing transcribe RNA to cDNA and analyzing by qPCR or sequencing.

RNA Pull-Down

Fig. 2 Schematic diagram of RNA pull-down technology.

• RNA pull-down is a popular RNA-centered method for studying RNA-protein interactions, which is mainly used to find proteins that bind to the target lncRNA.
• We help our customers analyze plant lncRNA interaction validation by RNA pull-down. First, we use de-sulfated biotin to label RNA ends and then use streptavidin magnetic beads to enrich RNA. In the second step, we add protein lysis solution to make RNA bind to protein, and in the third step, we wash the magnetic beads, separate the bindings, and finally detect the faint down protein.

EMSA

• RNA EMSA is a technique for detecting protein-RNA interactions by gel electrophoresis migration. Its principle is that labeled RNA probes and proteins are incubated. When protein-RNA complexes are formed, non-denaturing polyacrylamide gels are used to separate from determining the specificity of the RNA-bound protein.
• We help our customers analyze plant lncRNA interaction validation by EMSA. Generally, a conventional RNA EMSA protocol consists of three key steps: binding reactions, electrophoresis, and probe detection.

Lifeasible provides cost-effective, high-quality, and hassle-free services to our customers worldwide. We provide our clients with direct access to our experts and prompt responses to their questions. If you are interested in our services or have questions, please contact us or make an online inquiry.

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For research use only, not intended for any clinical use.

Related Services

• Plant lncRNA Expression Validation Analysis
• Plant lncRNA Localization Validation Analysis
• Plant lncRNA Non-Coding Ability Validation Analysis