Long non-coding RNA (lncRNA) with longer than 200 bp in length do not contain open reading frames, and they generally do not have a protein-coding function. They are ubiquitous in the body and play a variety of biological roles. Overexpression of lncRNA genes for gain-of-function experiments is an essential tool for studying gene function. In principle, full-length lncRNAs are targeted to be cloned into expression vectors to achieve lncRNA overexpression or different research strategies are adopted depending on the localization of lncRNAs on the genome.

Lifeasible provides a complete set of technical services, including lncRNA overexpression vector construction, plant transfection, gene expression assay, phenotyping of transgenic plants, and others, to help our customers worldwide in plant science research. Our platform is equipped with cutting-edge facilities and professional experts to support research. Here, we provide various services according to customers' demands.

Construction of lncRNA Overexpression Vector

• Gene overexpression to determine plant gene function has been used almost since the first plant transformation protocols became viable. These experiments aim to observe any phenotypic change associated with changing the expression of a gene of interest in this case overexpression.
• We help customers to construct efficient lncRNA overexpression plasmids. Our workflow includes cloning primer design, target fragment amplification, preparation of linearized overexpression vectors, recombinant vectors, the transformation of E. coli, and sequencing analysis.

Plant Transfection

• The delivery and overexpression of genes in plant cells have been particularly interesting to plant research and biotechnology.
• According to the demand of customer research, we provide plant transfection with a lncRNA overexpression vector in several plants, such as Arabidopsis, tobacco, rice, barley, potato, kale, etc. Here, we present our workflow with the example of Arabidopsis thaliana, mainly Agrobacterium tumefaciens preparation and infiltration of Arabidopsis plants' inflorescence.

Gene Expression Assays & Screening of Overexpression Plants

Fig. 1 Schematic diagram of screening overexpression plants.

Gene expression analysis is most simply described as the study of the way genes are transcribed to synthesize functional gene products. We help customers with gene expression assays by real-time polymerase chain reaction (RT-PCR) and Northern blot methods, which also allow the identification of overexpressed plants at the molecular level.

Phenotyping of Transgenic Plants

Finally, we phenotypically characterized the overexpression plants to determine the effects of lncRNA on plant growth and development, such as measuring seed germination rate, root length, plant fresh weight, dry weight, etc.

Lifeasible provides cost-effective, high-quality, and hassle-free services to our customers worldwide. We provide our clients with direct access to our experts and prompt responses to their questions. If you are interested in our services or have questions, please contact us or make an online inquiry.

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Related Services

• Plant lncRNA Loss-Of-Function Analysis
• Functional Analysis of Plant lncRNA with CRISPR-Cas Technology
• Plant lncRNA Other Knockdown Analysis