Embryo culture refers to the technique of isolated culture of embryo, ovary, ovule, and endosperm of plants to develop them into complete plants. Embryo culture can overcome the sterility of distant hybrids Embryo culture and in vitro fertilization techniques are used to solve the problem of interspecific and intergeneric embryo failure, incomplete embryo development, and failure to germinate properly. In addition, the use of embryo culture technology can make plants with incomplete embryo development obtain offspring, such as orchids, asparagus seed maturity, embryo only 6 to 7 cells, most embryos cannot be viable, isolate these immature embryos for culture, you can get normal plants. The application of embryo culture technology can also shorten the breeding cycle.

What We Offer

Lifeasible offers a variety of plant embryo culture methods, including embryo culture (mature embryo culture, young embryo culture), ovule culture, ovary culture, endosperm culture, and in vitro pollination.

Mature embryo culture

• Mature embryo refers to the embryo after the cotyledon stage until it is fully developed. It is easier to cultivate success and can grow normally into seedlings on a medium containing inorganic and abundant elements and sugar.

Young embryo culture

• Young embryos are young embryos before the cotyledon stage, and the smaller the embryo the more difficult it is to culture, but Lifeasible can now culture heart-shaped stage embryos or earlier (0.1 to 0.2 mm) and we have had success in culturing young embryos of barley, shepherd's purse, sugar cane, sugar beet, carrot, etc.

Culture essential substances

• The media we commonly use are Tukey, MS, B5, and Nitsch media.
• The added substances we prepare are sucrose, IAA (to increase the length of embryos), BA (to improve the chances of embryo survival), coconut milk, endosperm extract of melon (to promote embryo growth), amino acids, and inorganic salts.

We Do Better

Lifeasible, as a leading plant technology company, has rich experience and mature technology in plant embryo culture. We have obtained successful cases in embryo culture of citrus, grapefruit, lemon, lime, loquat, longan, peach, grape, kiwi, rice, wheat, barley, maize, sorghum, apple, and many other plants, and we are your reliable technical service partner.

Reference

1. Seyed J. D., et al., “Micropropagation of common yew using Embryo culture.” Journal of Applied Biotechnology Reports, 2014, 1(2): 81-84.
2. Dayarani M., et al., “Embryo culture and embryo rescue studies in wild Musa spp. (Musa ornata).” Journal of Applied Horticulture, 2014, 16(2): 126-130.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

For research use only, not intended for any clinical use.

Related Services

• Meristem Tissue Culture
• Anther Culture
• Microspore Culture
• Ovule Culture
• Callus Culture
• Protoplast Isolation and Culture